Rabbit SMAD6 Polyclonal Antibody | anti-SMAD6 antibody

Rabbit Polyclonal (IgG) to Human SMAD6

Cat. Num. AAA241862

• Gene Names: SMAD6; AOVD2; MADH6; MADH7; HsT17432
• Reactivity: Human, Pig; Predicted Reactivity: Bovine, Dog (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Western Blot
• Purity: Protein G Purified
• Synonyms: SMAD6; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human SMAD6; Anti-SMAD6 Antibody (aa85-99) IHC-plus; AOVD2; HSMAD6; HsT17432; SMAD 6; MADH7; Mothers against DPP homolog 6; SMAD family member 6; MAD homolog 6; MADH6; Human SMAD6; anti-SMAD6 antibody

• Host: Rabbit
• Reactivity: Human, Pig; Predicted Reactivity: Bovine, Dog (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Synthetic peptides corresponding to amino acids 85-99 (RAGGPPRPMSEPGAG) and amino acids 372-388 (NLEQRSESVRRTRSKIG) of human SMAD6 (GenBank Accession No. AAH12986.1). These peptide sequences are specific to SMAD6, and have high homology to SMAD6in multiple species.
• Purity/Purification: Protein G Purified
• Form/Format: PBS, 0.05% BSA, 0.05% sodium azide.
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 496

• Applicable Applications for anti-SMAD6 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (5 ug/ml), WB (0.5 - 2 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: This antibody was developed against synthetic peptides corresponding to amino acids 85-99 (RAGGPPRPMSEPGAG)and amino acids 372-388 (NLEQRSESVRRTRSKIG)of human SMAD6 (GenBank Accession No. AAH12986.1).
• Immunogen Type: Synthetic peptide
• Immunogen: SMAD6 antibody was raised against this antibody was developed against synthetic peptides corresponding to amino acids 85-99 (RAGGPPRPMSEPGAG)and amino acids 372-388 (NLEQRSESVRRTRSKIG)of human SMAD6 (GenBank Accession No. AAH12986.1). These peptide sequences are specific to SMAD6, and have high homology to SMAD6in multiple species. Percent identity by BLAST analysis: Human, Pig (100%); Dog, Bovine, Opossum (93%); Gibbon, Marmoset, Rat (87%); Elephant (80%).
• Antigen Modification: aa85-99
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-SMAD6 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 92859871
• NCBI GeneID: 4091
• NCBI Accession #: NP_005576.3
• NCBI GenBank Nucleotide #: NM_005585.4
• UniProt Accession #: O43541; O43654; Q15799; Q7Z7L4; Q96E31; Q9UKZ3; A9J6M5
• Molecular Weight: 35,467 Da
• NCBI Official Full Name: mothers against decapentaplegic homolog 6
• NCBI Official Synonym Full Names: SMAD family member 6
• NCBI Official Symbol: SMAD6
• NCBI Official Synonym Symbols: AOVD2; MADH6; MADH7; HsT17432
• NCBI Protein Information: mothers against decapentaplegic homolog 6; MAD homolog 6; SMAD, mothers against DPP homolog 6; mothers against decapentaplegic, drosophila, homolog of, 6
• UniProt Protein Name: Mothers against decapentaplegic homolog 6
• Protein Family: Mothers against decapentaplegic
• UniProt Gene Name: SMAD6
• UniProt Synonym Gene Names: MADH6; MAD homolog 6; Mothers against DPP homolog 6; SMAD 6; Smad6; hSMAD6
• UniProt Entry Name: SMAD6_HUMAN

NCBI Description

The protein encoded by this gene belongs to the SMAD family of proteins, which are related to Drosophila 'mothers against decapentaplegic' (Mad) and C. elegans Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions in the negative regulation of BMP and TGF-beta/activin-signalling. Multiple transcript variants have been found for this gene.[provided by RefSeq, Sep 2014]

Uniprot Description

SMAD6: Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit selectively BMP (bone morphogenetic proteins) signaling by competing with the co-SMAD SMAD4 for receptor-activated SMAD1. SMAD6 is an inhibitory SMAD (I-SMAD) or antagonistic SMAD. Binds to regulatory elements in target promoter regions. Interacts with NEDD4L. Interacts with WWP1. Interacts with RNF111 and AXIN1. Interacts with TGF-beta type I receptor superfamily members, SMAD1, HOXC8 and HOXC9. Interacts with STAMBP and PRKX. Ubiquitous in various organs, with higher levels in lung. Isoform B is up-regulated in diseased heart tissue. Belongs to the dwarfin/SMAD family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 15q22.31

Cellular Component: transcription factor complex; protein complex; cytoplasm; nucleus; cytosol

Molecular Function: identical protein binding; protein binding; metal ion binding; ubiquitin protein ligase binding; transforming growth factor beta receptor, inhibitory cytoplasmic mediator activity; chromatin binding; transcription factor activity

Biological Process: fat cell differentiation; BMP signaling pathway; negative regulation of cell proliferation; zygotic determination of dorsal/ventral axis; transcription, DNA-dependent; response to estrogen stimulus; ureteric bud development; transforming growth factor beta receptor signaling pathway; immune response; negative regulation of transforming growth factor beta receptor signaling pathway; cell-substrate adhesion; negative regulation of BMP signaling pathway; negative regulation of apoptosis

Disease: Aortic Valve Disease 2