Rabbit anti-Human SMAD5 Polyclonal Antibody | anti-SMAD5 antibody

SMAD5, ID (SMAD5, MADH5, Mothers against decapentaplegic homolog 5, JV5-1, SMAD family member 5)

Cat. Num. AAA6004192

• Gene Names: SMAD5; DWFC; JV5-1; MADH5
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: SMAD5; Polyclonal Antibody; ID (SMAD5; MADH5; Mothers against decapentaplegic homolog 5; JV5-1; SMAD family member 5); Anti -SMAD5; anti-SMAD5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-SMAD5 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500
• Immunogen: SMAD5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 241-269 amino acids from the Central region of human SMAD5.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(SMAD5 Antibody (Center) pre-incubated without(lane 1) and with(lane 2) blocking peptide in Hela cell line lysate. SMAD5 Antibody (Center) (arrow) was detected using the purified Pab.)

Related Product Information for anti-SMAD5 antibody

Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).

Product Categories/Family for anti-SMAD5 antibody

Antibodies; Abs to Smad Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 2282612
• NCBI GeneID: 4090
• UniProt Accession #: Q99717; O14688; Q15798; Q9UQA1
• Molecular Weight: 52,258 Da
• NCBI Official Full Name: SMAD5
• NCBI Official Synonym Full Names: SMAD family member 5
• NCBI Official Symbol: SMAD5
• NCBI Official Synonym Symbols: DWFC; JV5-1; MADH5
• NCBI Protein Information: mothers against decapentaplegic homolog 5; SMA- and MAD-related protein 5; SMAD, mothers against DPP homolog 5; MAD, mothers against decapentaplegic homolog 5; mothers against decapentaplegic, drosophila, homolog of, 5
• UniProt Protein Name: Mothers against decapentaplegic homolog 5
• Protein Family: SMAD5 antisense gene protein
• UniProt Gene Name: SMAD5
• UniProt Synonym Gene Names: MADH5; MAD homolog 5; Mothers against DPP homolog 5; SMAD 5; Smad5; hSmad5
• UniProt Entry Name: SMAD5_HUMAN

NCBI Description

The protein encoded by this gene is involved in the TGF-beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by BMP type 1 receptor kinase. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

SMAD5: transcription factor phosphorylated and activated by bone morphogenetic protein receptor kinases. Phosphorylated Smads dimerize with collaborating Smad4 and are translocated into the nucleus, where they regulate transcription of target genes. Participates in a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation and apoptosis.

Protein type: DNA-binding; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: nucleoplasm; transcription factor complex; protein complex; cytoplasm; integral to membrane; intracellular; cytosol; nucleus

Molecular Function: protein binding; metal ion binding; ubiquitin protein ligase binding; receptor signaling protein activity; transcription factor activity; transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity

Biological Process: Mullerian duct regression; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; embryonic pattern specification; signal transduction; protein amino acid phosphorylation; germ cell development; BMP signaling pathway; positive regulation of osteoblast differentiation; ureteric bud development; transforming growth factor beta receptor signaling pathway; cartilage development; erythrocyte differentiation; cardiac muscle contraction; osteoblast fate commitment