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Immunofluorescence (IF)
(Immunofluorescence staining of SMAD2 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-mouse SMAD2 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red)Positive staining was localized to nucleus.)
Rabbit anti-Mouse Smad2 Polyclonal Antibody | anti-Smad2 antibody
Anti-Smad2 Antibody, Rabbit Polyclonal
Gene Names
Smad2; Madh2; Madr2; mMad2; Smad-2; 7120426M23Rik
Reactivity
Mouse
Applications
Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Purity
Protein A & Antigen Affinity
Synonyms
Smad2; Polyclonal Antibody; Anti-Smad2 Antibody; Rabbit Polyclonal; Smad2 Antibody; Rabbit PAb; Antigen Affinity Purified; SMAD family member 2; Anti-7120426M23Rik Antibody; Anti-Madh2 Antibody; Anti-Madr2 Antibody; Anti-mMad2 Antibody; Anti-Smad-2 Antibody; anti-Smad2 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Mouse Smad2
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-Smad2 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:2000
ELISA: 1:5000-1:10,000
IHC-P: 1:250-1:1000
ICC/IF: 1:300-1:10,000
ELISA: 1:5000-1:10,000
IHC-P: 1:250-1:1000
ICC/IF: 1:300-1:10,000
Immunogen
Recombinant Mouse Smad2 Protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Mouse Smad2 (rM Smad2). Smad2 specific IgG was purified by Mouse Smad2 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunofluorescence (IF)
(Immunofluorescence staining of SMAD2 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-mouse SMAD2 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red)Positive staining was localized to nucleus.)
Immunofluorescence (IF)
(Immunofluorescence staining of SMAD2 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-mouse SMAD2 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red)Positive staining was localized to nucleus.)
Immunohistochemistry (IHC)
(Immunochemical staining of mouse SMAD2 in mouse skeletal muscle with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of mouse SMAD2 in mouse skeletal muscle with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of mouse SMAD2 in mouse heart with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of mouse SMAD2 in mouse heart with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of mouse SMAD2 in mouse brain with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of mouse SMAD2 in mouse brain with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
Western Blot (WB)
(Anti-SMAD2 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: A431 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:58 kDaObserved band size:58 kDa)
Western Blot (WB)
(Anti-SMAD2 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: A431 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:58 kDaObserved band size:58 kDa)
Related Product Information for anti-Smad2 antibody
SMAD2 is a member of the SMAD family. Members of this family mediate signal transduction by the TGF-beta/activin/BMP-2/4 cytokine superfamily from receptor Ser/Thr protein kinases at the cell surface to the nucleus. SMAD2 mediates the signal of the TGF-beta, and therefore regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. SMAD2 is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. SMAD2 is the downstream signal transducers of TGF-beta-1 in human dental pulp cells. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. Phosphorylated SMAD2 is able to form a complex with SMAD4 or SARA. These complexes accumulate in the cell nucleus, where they are directly participating in the regulation of gene expression.
Product Categories/Family for anti-Smad2 antibody
References
Feng. et al., 2002, Mol Cell. 9 (1): 133-43. Zhu Y. et al., 1997, J Biol Chem. 272 (15): 10035-40. Zi Z. et al., 2012, FEBS Lett. 586 (14): 1921-8.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
48,956 Da
NCBI Official Full Name
mothers against decapentaplegic homolog 2 isoform 1
NCBI Official Synonym Full Names
SMAD family member 2
NCBI Official Symbol
Smad2
NCBI Official Synonym Symbols
Madh2; Madr2; mMad2; Smad-2; 7120426M23Rik
NCBI Protein Information
mothers against decapentaplegic homolog 2
UniProt Protein Name
Mothers against decapentaplegic homolog 2
Protein Family
UniProt Gene Name
Smad2
UniProt Synonym Gene Names
Madh2; Madr2; MAD homolog 2; Mothers against DPP homolog 2; mMad2; SMAD 2; Smad2
Uniprot Description
Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator ().
Research Articles on Smad2
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Product Notes
The Smad2 smad2 (Catalog #AAA8112007) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Smad2 Antibody, Rabbit Polyclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Smad2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF). WB: 1:500-1:2000 ELISA: 1:5000-1:10,000 IHC-P: 1:250-1:1000 ICC/IF: 1:300-1:10,000. Researchers should empirically determine the suitability of the Smad2 smad2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Smad2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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