Rabbit anti-Human, Mouse Smad Ubiquitination Regulatory Factor-1 Polyclonal Antibody | anti-SMURF1 antibody
Smad Ubiquitination Regulatory Factor-1 (Smurf1)
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
Smad Ubiquitination Regulatory Factor-1; Polyclonal Antibody; Smad Ubiquitination Regulatory Factor-1 (Smurf1); Anti -Smad Ubiquitination Regulatory Factor-1 (Smurf1); anti-SMURF1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
Reacts with human and mouse Smurf1. On Western blots, it identifies the target band at ~100kD. Reactivity has been confirmed with human A375, NGP96, HepG2, MeWo cell lysates, and mouse liver and testis homogenates. Based on amino acid sequence homology, reactivity with rat may also be observed.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Applicable Applications for anti-SMURF1 antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot: 1-3ug/ml
Optimal dilutions to be determined by the researcher
Dilution: Western Blot: 1-3ug/ml
Optimal dilutions to be determined by the researcher
Immunogen
Synthetic peptide derived from an internal region of human Smurf1 (Smad ubiquitination regulatory factor 1, Smad-specific E3 ubiquitin ligase 1, Smad-ubiquitin E3 ligase Smurf1-beta).
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-SMURF1 antibody
Smurf1 (Smad ubiquitination regulatory factor-1) is a member of the Hect family of proteins, which also includes the ubiquitin (Ub) E3-type ligases Nedd3 and E6-AP. E3 ligases are involved in the enzymatic reactions of the Ub conjugating pathway that targets proteins for degradation by the 26S proteasome. In the Ub pathway, E3 ligases catalyze the transfer of Ub from Ub-conjugating enzymes to the individual protein substrate. As an E3 ligase, Smurf1 preferentially promotes the transfer of Ub to Smad proteins, thereby targeting them for degradation. Smurf1 regulates the inhibitory activity of Smad by targeting it to the plasma membrane, and is also known to enhance the degradation of the inhibitory Smad1 and Smad5, thereby down-regulating the BMP pathway, which controls embryomic development and the specification of ectoderm- and mesoderm-derived tissues.
Product Categories/Family for anti-SMURF1 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The SMURF1 (Catalog #AAA614685) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Smad Ubiquitination Regulatory Factor-1 (Smurf1) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Smad Ubiquitination Regulatory Factor-1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 1-3ug/ml Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the SMURF1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Smad Ubiquitination Regulatory Factor-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
