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Western Blot (WB) (Western blot analysis of TAGLN antibody (C-term) in mouse bladder tissue lysates (35ug/lane). TAGLN (arrow) was detected using the purified Pab.)

Rabbit anti-Human, Mouse SM22 alpha Polyclonal Antibody | anti-TAGLN antibody

SM22 alpha, CT (Transgelin, Smooth Muscle Protein 22-alpha, TAGLN, Protein WS3-10, 22kD Actin-binding Protein, WS3-10, SM22) (MaxLight 550)

Gene Names
TAGLN; SM22; SMCC; TAGLN1; WS3-10
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Purified by Ammonium Sulfate Precipitation.
Synonyms
SM22 alpha; Polyclonal Antibody; CT (Transgelin; Smooth Muscle Protein 22-alpha; TAGLN; Protein WS3-10; 22kD Actin-binding Protein; WS3-10; SM22) (MaxLight 550); anti-TAGLN antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human TAGLN. Species Crossreactivity: mouse.
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.
Applicable Applications for anti-TAGLN antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:50-1:100
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the C-terminal region of human TAGLN (KLH).
Conjugate
MaxLight550
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of TAGLN antibody (C-term) in mouse bladder tissue lysates (35ug/lane). TAGLN (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of TAGLN antibody (C-term) in mouse bladder tissue lysates (35ug/lane). TAGLN (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human skeletal muscle reacted with TAGLN Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human skeletal muscle reacted with TAGLN Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(TAGLN Antibody (C-term) flow cytometric analysis of NCI-H292 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (TAGLN Antibody (C-term) flow cytometric analysis of NCI-H292 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-TAGLN antibody
References
1. Zhao, L., Mod. Pathol. 22(6), 786-796 (2009). 2. Leguillette, R., Am. J. Respir. Crit. Care Med. 179(3), 194-204 (2009). 3. Yu, H., FASEB J. 22(6), 1778-1789 (2008).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
22,611 Da
NCBI Official Full Name
transgelin
NCBI Official Synonym Full Names
transgelin
NCBI Official Symbol
TAGLN
NCBI Official Synonym Symbols
SM22; SMCC; TAGLN1; WS3-10
NCBI Protein Information
transgelin
UniProt Protein Name
Transgelin
Protein Family
UniProt Gene Name
TAGLN
UniProt Synonym Gene Names
SM22; WS3-10; SM22-alpha
UniProt Entry Name
TAGL_HUMAN

NCBI Description

The protein encoded by this gene is a transformation and shape-change sensitive actin cross-linking/gelling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

TAGLN: Actin cross-linking/gelling protein. Involved in calcium interactions and contractile properties of the cell that may contribute to replicative senescence. Belongs to the calponin family.

Protein type: Nuclear receptor co-regulator; Motility/polarity/chemotaxis; Actin-binding

Chromosomal Location of Human Ortholog: 11q23.2

Cellular Component: cytoplasm

Molecular Function: actin filament binding; protein binding

Biological Process: muscle development; epithelial cell differentiation

Research Articles on TAGLN

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Product Notes

The TAGLN tagln (Catalog #AAA6499673) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SM22 alpha, CT (Transgelin, Smooth Muscle Protein 22-alpha, TAGLN, Protein WS3-10, 22kD Actin-binding Protein, WS3-10, SM22) (MaxLight 550) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SM22 alpha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:50-1:100 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the TAGLN tagln for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SM22 alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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