Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Mouse Slit Homolog 3 (Slit3) Polyclonal Antibody | anti-Slit3 antibody
Polyclonal Antibody to Slit Homolog 3 (Slit3)
Gene Names
Slit3; Slil2; Slit1; b2b2362.1Clo
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Slit Homolog 3 (Slit3); Polyclonal Antibody; Polyclonal Antibody to Slit Homolog 3 (Slit3); anti-Slit3 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against Slit3. It has been selected for its ability to recognize Slit3 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.
MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- KDS VVCECHPGWT GPLCDQEARD PCLGHSCRHG TCMATGDSYV CKCAEGYGGA LCDQKNDSAS ACSAFKCHHG QCHISDRGEP YCLCQPGFSG HHCEQENPCM GEIVREAIRR QKDYASCATA SKVPIMECRG GCGSQCCQPI RSKRRKYVFQ CTDGSSFVEE VERHLEC
MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- KDS VVCECHPGWT GPLCDQEARD PCLGHSCRHG TCMATGDSYV CKCAEGYGGA LCDQKNDSAS ACSAFKCHHG QCHISDRGEP YCLCQPGFSG HHCEQENPCM GEIVREAIRR QKDYASCATA SKVPIMECRG GCGSQCCQPI RSKRRKYVFQ CTDGSSFVEE VERHLEC
Sequence Length
1523
Applicable Applications for anti-Slit3 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant Slit3 (Lys1348~Cys1517) expressed in E.coli.
Cross Reactivity
Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2071086
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Stomach Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Stomach Tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
167,727 Da
NCBI Official Full Name
slit homolog 3 protein
NCBI Official Synonym Full Names
slit guidance ligand 3
NCBI Official Symbol
Slit3
NCBI Official Synonym Symbols
Slil2; Slit1; b2b2362.1Clo
NCBI Protein Information
slit homolog 3 protein
UniProt Protein Name
Slit homolog 3 protein
Protein Family
UniProt Gene Name
Slit3
UniProt Synonym Gene Names
Slit-3; Slit3
Uniprot Description
SLIT3: May act as molecular guidance cue in cellular migration, and function may be mediated by interaction with roundabout homolog receptors. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 11|11 A4
Cellular Component: extracellular region; extracellular space; membrane; mitochondrion
Molecular Function: calcium ion binding; receptor binding; Roundabout binding
Biological Process: axon extension involved in axon guidance; axon guidance; cell differentiation; multicellular organism development; negative chemotaxis; negative regulation of cell growth; negative regulation of cell proliferation; nervous system development; organ morphogenesis
Research Articles on Slit3
Similar Products
Product Notes
The Slit3 slit3 (Catalog #AAA2004224) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Slit Homolog 3 (Slit3) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Slit Homolog 3 (Slit3) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the Slit3 slit3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- KDS VVCECHPGWT GPLCDQEARD PCLGHSCRHG TCMATGDSYV CKCAEGYGGA LCDQKNDSAS ACSAFKCHHG QCHISDRGEP YCLCQPGFSG HHCEQENPCM GEIVREAIRR QKDYASCATA SKVPIMECRG GCGSQCCQPI RSKRRKYVFQ CTDGSSFVEE VERHLEC. It is sometimes possible for the material contained within the vial of "Slit Homolog 3 (Slit3), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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