Goat SLIMMER/FHL1 isoform B Polyclonal Antibody | anti-FHL1 antibody

Goat anti-SLIMMER/FHL1 isoform B Antibody

Cat. Num. AAA421232

• Gene Names: FHL1; KYOT; SLIM; FHL-1; FHL1A; FHL1B; FLH1A; SLIM1; XMPMA; RBMX1A; RBMX1B; SLIM-1; SLIMMER
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse, Dog
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: SLIMMER/FHL1 isoform B; Polyclonal Antibody; Goat anti-SLIMMER/FHL1 isoform B Antibody; Goat Anti-FHL1 / SLIM1; Biotinylated Antibody; FHL1; KYO-T; SLIM1; bA535K18.1; four and a half LIM domains 1; Four-and-a-half LIM domains 1; bA535K18.1 (LIM protein SLIMMER); RP11-535K18.1; FHL1A; FHL1B; FLH1A; KYOT; MGC111107; SLIMMER; XMPMA; LIM protein SLIMMER; four-and-a-half Lin11; Isl-1 and Mec-; FHL1 antibody; KYO-T antibody; SLIM1 antibody; bA535K18.1 antibody; four and a half LIM domains 1 antibody; Four-and-a-half LIM domains 1 antibody; bA535K18.1 (LIM protein SLIMMER) antibody; RP11-535K18.1 antibody; FHL1A antibody; FHL1B antibody; FLH1A antibody; KYOT antibody; MGC111107 antibody; antibody; XMPMA antibody; LIM protein SLIMMER antibody; four-and-a-half Lin11 antibody; Isl-1 and Mec-3 domains 1 antibody; skeletal muscle LIM-protein1 antibody; SLIMMER / FHL1 isoform B; anti-FHL1 antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse, Dog
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: CTVSRVSHPVSKARK
• Sequence Length: 323

• Applicable Applications for anti-FHL1 antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 128000.; Western Blot: Approx 30kDa band observed in Human Heart lysates is consistent with literature (PMID: 10480922; calculated MW of 36.3kDa according to AAC72886). Recommended concentration: 0. 01-0.1ug/ml.
• Immunogen: Peptide with sequence CTVSRVSHPVSKARK, from the internal region of the protein sequence according to AAC72886.1.
• Epitope: Internal region
• Note: This antibody is specific for the FHL-1 isoform B (SLIMMER) and is expected to recognise an epitope corresponding to aa 233-246 of human SLIMMER protein.
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0. 01ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 3859849
• NCBI GeneID: 2273
• Molecular Weight: 33,579 Da
• NCBI Official Full Name: LIM protein SLIMMER
• NCBI Official Synonym Full Names: four and a half LIM domains 1
• NCBI Official Symbol: FHL1
• NCBI Official Synonym Symbols: KYOT; SLIM; FHL-1; FHL1A; FHL1B; FLH1A; SLIM1; XMPMA; RBMX1A; RBMX1B; SLIM-1; SLIMMER
• NCBI Protein Information: four and a half LIM domains protein 1
• UniProt Protein Name: Four and a half LIM domains protein 1
• Protein Family: Pre-rRNA-processing protein
• UniProt Gene Name: FHL1
• UniProt Synonym Gene Names: SLIM1; FHL-1; SLIM; SLIM-1
• UniProt Entry Name: FHL1_HUMAN

NCBI Description

This gene encodes a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. Expression of these family members occurs in a cell- and tissue-specific mode and these proteins are involved in many cellular processes. Mutations in this gene have been found in patients with Emery-Dreifuss muscular dystrophy. Multiple alternately spliced transcript variants which encode different protein isoforms have been described.[provided by RefSeq, Nov 2009]

Uniprot Description

May have an involvement in muscle development or hypertrophy.