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Western Blot (WB) (Western blot analysis of extracts from RAW264.7 cells using SLC9A9 antibody.The lane on the left is treated with the antigen-specific peptide.)

Rabbit anti-Human, Mouse SLC9A9 Polyclonal Antibody | anti-SLC9A9 antibody

SLC9A9 Antibody

Gene Names
SLC9A9; NHE9; AUTS16
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
SLC9A9; Polyclonal Antibody; SLC9A9 Antibody; 5730527A11Rik; 9930105B05; AI854429; FLJ35613; Na(+)/H(+) exchanger 9; Nbla00118; NHE 9; NHE-9; NHE9; Putative protein product of Nbla00118; SL9A9_HUMAN; Slc9a9; Sodium/hydrogen exchanger 9; Sodium/proton exchanger NHE9; Solute carrier family 9 (sodium/hydrogen exchanger) isoform 9; Solute carrier family 9 (sodium/hydrogen exchanger) member 9; Solute carrier family 9 member 9; anti-SLC9A9 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
SLC9A9 antibody detects endogenous levels of total SLC9A9
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
645
Applicable Applications for anti-SLC9A9 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Subcellular Location
Endosome
Tissue Specificity
Ubiquitously expressed in all tissues tested. Expressed at highest levels in heart and skeletal muscle, followed by placenta, kidney, and liver. Expressed in the brain, in the medulla and spinal cord.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog
Similarity
Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from RAW264.7 cells using SLC9A9 antibody.The lane on the left is treated with the antigen-specific peptide.)

Western Blot (WB) (Western blot analysis of extracts from RAW264.7 cells using SLC9A9 antibody.The lane on the left is treated with the antigen-specific peptide.)

Immunofluorescene (IF)

(MBS9605974 staining A549 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

Immunofluorescene (IF) (MBS9605974 staining A549 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

Western Blot (WB)

(Western blot analysis of extracts from various samples, using SLC9A9 Antibody. Lane 1: 293 treated with blocking peptide. Lane 2: 293; Lane 3: Hela; )

Western Blot (WB) (Western blot analysis of extracts from various samples, using SLC9A9 Antibody. Lane 1: 293 treated with blocking peptide. Lane 2: 293; Lane 3: Hela; )
Related Product Information for anti-SLC9A9 antibody
Function: May act in electroneutral exchange of protons for Na+ across membranes. Involved in the effusion of Golgi luminal H+ in exchange for cytosolic cations. Involved in organelle ion homeostasis by contributing to the maintenance of the unique acidic pH values of the Golgi and post-Golgi compartments in the cell.
Similarity: Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 65 kD
Predicted: 73 kDa
NCBI Official Full Name
sodium/hydrogen exchanger 9
NCBI Official Synonym Full Names
solute carrier family 9 member A9
NCBI Official Symbol
SLC9A9
NCBI Official Synonym Symbols
NHE9; AUTS16
NCBI Protein Information
sodium/hydrogen exchanger 9
UniProt Protein Name
Sodium/hydrogen exchanger 9
Protein Family
UniProt Gene Name
SLC9A9
UniProt Synonym Gene Names
NHE9; NHE-9

NCBI Description

This gene encodes a sodium/proton exchanger that is a member of the solute carrier 9 protein family. The encoded protein localizes the to the late recycling endosomes and may play an important role in maintaining cation homeostasis. Mutations in this gene are associated with autism susceptibility 16 and attention-deficit/hyperactivity disorder. [provided by RefSeq, Mar 2012]

Uniprot Description

May act in electroneutral exchange of protons for Na+ across membranes. Involved in the effusion of Golgi luminal H+ in exchange for cytosolic cations. Involved in organelle ion homeostasis by contributing to the maintenance of the unique acidic pH values of the Golgi and post-Golgi compartments in the cell.

Research Articles on SLC9A9

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Product Notes

The SLC9A9 slc9a9 (Catalog #AAA9605974) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SLC9A9 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SLC9A9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the SLC9A9 slc9a9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SLC9A9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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