Rabbit anti-Human SLC8A1 Polyclonal Antibody | anti-SLC8A1 antibody

SLC8A1 Polyclonal Antibody

Cat. Num. AAA9132794

• Gene Names: SLC8A1; NCX1
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purification
• Synonyms: SLC8A1; Polyclonal Antibody; SLC8A1 Polyclonal Antibody; NCX1; anti-SLC8A1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Sequence Length: 973

• Applicable Applications for anti-SLC8A1 antibody: Western Blot (WB)
• Application Notes: WB: 1:1000 - 1:2000
• Immunogen: Recombinant protein of human SLC8A1
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using SLC8A1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)

Related Product Information for anti-SLC8A1 antibody

In cardiac myocytes, Ca(2+) concentrations alternate between high levels during contraction and low levels during relaxation. The increase in Ca(2+) concentration during contraction is primarily due to release of Ca(2+) from intracellular stores. However, some Ca(2+) also enters the cell through the sarcolemma (plasma membrane). During relaxation, Ca(2+) is sequestered within the intracellular stores. To prevent overloading of intracellular stores, the Ca(2+) that entered across the sarcolemma must be extruded from the cell. The Na(+)-Ca(2+) exchanger is the primary mechanism by which the Ca(2+) is extruded from the cell during relaxation. In the heart, the exchanger may play a key role in digitalis action. The exchanger is the dominant mechanism in returning the cardiac myocyte to its resting state following excitation.

Product Categories/Family for anti-SLC8A1 antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 12644210
• NCBI GeneID: 6546
• UniProt Accession #: P32418; O95849; Q4QQG6; Q587I6; Q59GN4; Q9UBL8; Q9UD55; Q9UDN1; Q9UDN2; Q9UKX6; A8K6N1; D6W595
• Molecular Weight: 109kDa
• NCBI Official Full Name: Sodium/calcium exchanger 1
• NCBI Official Synonym Full Names: solute carrier family 8 member A1
• NCBI Official Symbol: SLC8A1
• NCBI Official Synonym Symbols: NCX1
• NCBI Protein Information: sodium/calcium exchanger 1
• UniProt Protein Name: Sodium/calcium exchanger 1
• Protein Family: Sodium/calcium exchanger
• UniProt Gene Name: SLC8A1

NCBI Description

In cardiac myocytes, Ca(2+) concentrations alternate between high levels during contraction and low levels during relaxation. The increase in Ca(2+) concentration during contraction is primarily due to release of Ca(2+) from intracellular stores. However, some Ca(2+) also enters the cell through the sarcolemma (plasma membrane). During relaxation, Ca(2+) is sequestered within the intracellular stores. To prevent overloading of intracellular stores, the Ca(2+) that entered across the sarcolemma must be extruded from the cell. The Na(+)-Ca(2+) exchanger is the primary mechanism by which the Ca(2+) is extruded from the cell during relaxation. In the heart, the exchanger may play a key role in digitalis action. The exchanger is the dominant mechanism in returning the cardiac myocyte to its resting state following excitation.[supplied by OMIM, Apr 2004]

Uniprot Description

NCX1: Rapidly transports Ca(2+) during excitation-contraction coupling. Ca(2+) is extruded from the cell during relaxation so as to prevent overloading of intracellular stores. Belongs to the sodium/potassium/calcium exchanger family. SLC8 subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Motility/polarity/chemotaxis; Transporter; Transporter, SLC family

Chromosomal Location of Human Ortholog: 2p22.1

Cellular Component: integral to plasma membrane; nucleoplasm; plasma membrane; sarcolemma; T-tubule; Z disc

Molecular Function: ankyrin binding; calcium ion binding; calcium:sodium antiporter activity; cytoskeletal protein binding; protein binding

Biological Process: calcium ion homeostasis; cardiac muscle cell development; cardiac muscle contraction; cellular sodium ion homeostasis; ion transport; muscle contraction; positive regulation of bone mineralization; reduction of cytosolic calcium ion concentration; regulation of heart rate; regulation of the force of heart contraction; vascular smooth muscle contraction