Rabbit SLC6A6 / Taurine Transporter Polyclonal Antibody | anti-SLC6A6 antibody

Rabbit Polyclonal (IgG) to Human SLC6A6 / Taurine Transporter

Cat. Num. AAA243175

• Gene Names: SLC6A6; TAUT
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: Immunoaffinity Purified
• Synonyms: SLC6A6 / Taurine Transporter; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human SLC6A6 / Taurine Transporter; Anti-SLC6A6 / Taurine Transporter Antibody (aa561-610) IHC-plus; SLC6A6; TAUT; Taurine transporter; Human SLC6A6; Taurine Transporter; anti-SLC6A6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SLC6A6 Antibody detects endogenous levels of total SLC6A6 protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 620

• Applicable Applications for anti-SLC6A6 antibody: Immunohistochemistry (IHC - Paraffin), Peptide Enzyme-Linked Immunosorbent Assay
• Application Notes: IHC-P (1:200), Peptide Enzyme-Linked Immunosorbent Assay (1:10000); ; optimal dilution to be deterined by the researcher)
• Target Species: Human
• Immunogen: SLC6A6 / Taurine Transporter antibody was raised against synthetic peptide from human SLC6A6 (aa561-610).
• Epitope: aa561-610
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-SLC6A6 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Product Categories/Family for anti-SLC6A6 antibody

Family: Transporter; Subfamily: Neurotransmitter:sodium symporter

NCBI and Uniprot Product Information

• NCBI GI #: 54607094
• NCBI GeneID: 6533
• NCBI Accession #: NP_003034.2
• NCBI GenBank Nucleotide #: NM_003043.5
• UniProt Accession #: P31641; Q9BRI2; Q9BXB0; B2RNU7; AAB25509
• Molecular Weight: Western Blot: Predicted MW 69 kDa
• NCBI Official Full Name: sodium- and chloride-dependent taurine transporter isoform a
• NCBI Official Synonym Full Names: solute carrier family 6 (neurotransmitter transporter), member 6
• NCBI Official Symbol: SLC6A6
• NCBI Official Synonym Symbols: TAUT
• NCBI Protein Information: sodium- and chloride-dependent taurine transporter; solute carrier family 6 (neurotransmitter transporter, taurine), member 6
• UniProt Protein Name: Sodium- and chloride-dependent taurine transporter
• Protein Family: Sodium- and chloride-dependent taurine transporter
• UniProt Gene Name: SLC6A6
• UniProt Entry Name: SC6A6_HUMAN

NCBI Description

This gene encodes a multi-pass membrane protein that is a member of a family of sodium and chloride-ion dependent transporters. The encoded protein transports taurine and beta-alanine. There is a pseudogene for this gene on chromosome 21. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]

Uniprot Description

TAUT: Required for the uptake of taurine. Transports both taurine and beta-alanine which requires sodium ions. Chloride ions are necessary for optimal uptake. Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A6 subfamily.

Protein type: Membrane protein, multi-pass; Transporter, SLC family; Transporter; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3p25.1

Cellular Component: integral to plasma membrane; integral to membrane; plasma membrane

Molecular Function: neurotransmitter:sodium symporter activity; taurine binding; taurine:sodium symporter activity

Biological Process: amino acid metabolic process; taurine transport; amino acid transport; transport; neurotransmitter transport; ion transport; transmembrane transport