Standard Curve (Sample)
Human Sarcoplasmic/endoplasmic reticulum calcium ATPase 2 (ATP2A2) ELISA Kit | ATP2A2 elisa kit
Human Sarcoplasmic/endoplasmic reticulum calcium ATPase 2 (ATP2A2) ELISA Kit
Gene Names
ATP2A2; DD; DAR; ATP2B; SERCA2
Reactivity
Human
Synonyms
Sarcoplasmic/endoplasmic reticulum calcium ATPase 2 (ATP2A2); N/A; Human Sarcoplasmic/endoplasmic reticulum calcium ATPase 2 (ATP2A2) ELISA Kit; ATP2A2 elisa kit
Reactivity
Human
Samples
Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for ATP2A2 elisa kit
Intended Uses: This ATP2A1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ATP2A1. This ELISA kit for research use only!
Principle of the Assay: ATP2A1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ATP2A1 antibody and an ATP2A1-HRP conjugate. The assay sample and buffer are incubated together with ATP2A1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substHumane for HRP enzyme. The product of the enzyme-substHumane reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATP2A1 concentHumanion since ATP2A1 from samples and ATP2A1-HRP conjugate compete for the anti-ATP2A1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ATP2A1 from the sample, fewer sites are left to bind ATP2A1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentHumanion of standards. The ATP2A1 concentHumanion in each sample is interpolated from this standard curve.
Principle of the Assay: ATP2A1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ATP2A1 antibody and an ATP2A1-HRP conjugate. The assay sample and buffer are incubated together with ATP2A1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substHumane for HRP enzyme. The product of the enzyme-substHumane reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATP2A1 concentHumanion since ATP2A1 from samples and ATP2A1-HRP conjugate compete for the anti-ATP2A1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ATP2A1 from the sample, fewer sites are left to bind ATP2A1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentHumanion of standards. The ATP2A1 concentHumanion in each sample is interpolated from this standard curve.
Product Categories/Family for ATP2A2 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
109,734 Da
NCBI Official Full Name
sarcoplasmic/endoplasmic reticulum calcium ATPase 2 isoform b
NCBI Official Synonym Full Names
ATPase, Ca++ transporting, cardiac muscle, slow twitch 2
NCBI Official Symbol
ATP2A2
NCBI Official Synonym Symbols
DD; DAR; ATP2B; SERCA2
NCBI Protein Information
sarcoplasmic/endoplasmic reticulum calcium ATPase 2; calcium pump 2; SR Ca(2+)-ATPase 2; cardiac Ca2+ ATPase; endoplasmic reticulum class 1/2 Ca(2+) ATPase; ATPase, Ca++ dependent, slow-twitch, cardiac muscle-2; calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform
UniProt Protein Name
Sarcoplasmic/endoplasmic reticulum calcium ATPase 2
UniProt Gene Name
ATP2A2
UniProt Synonym Gene Names
ATP2B; SERCA2; SR Ca(2+)-ATPase 2
UniProt Entry Name
AT2A2_HUMAN
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Product Notes
The Human ATP2A2 atp2a2 (Catalog #AAA247052) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA247052 ELISA Kit recognizes Human ATP2A2. It is sometimes possible for the material contained within the vial of "Sarcoplasmic/endoplasmic reticulum calcium ATPase 2 (ATP2A2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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