Mouse alpha-Mannosidase ELISA Kit | MAN2B1 elisa kit

Mouse alpha-Mannosidase ELISA Kit

Cat. Num. AAA247352

• Gene Names: MAN2B1; MANB; LAMAN
• Reactivity: Mouse
• Synonyms: alpha-Mannosidase; N/A; Mouse alpha-Mannosidase ELISA Kit; MAN2B1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of MANalpha. No significant cross-reactivity or interference between MANalpha and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MANalpha and all the analogues, therefore, cross reaction may still exist in some cases.
• Sequence Length: 987
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 ng/mL

• Reactivity Note: No significant cross-reactivity or interference between this antigen and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection, therefore, cross reaction may still exist in some cases.
• Preparation and Storage: Store at 2-8 degree C.; The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.

Standard Curve (Sample)

Related Product Information for MAN2B1 elisa kit

Principle of the Assay: MANalpha ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-MANalpha antibody and an MANalpha-HRP conjugate. The assay sample and buffer are incubated together with MANalpha-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MANalpha concentration since MANalpha from samples and MANalpha-HRP conjugate compete for the anti-MANalpha antibody binding site. Since the number of sites is limited, as more sites are occupied by MANalpha from the sample, fewer sites are left to bind MANalpha-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MANalpha concentration in each sample is interpolated from this standard curve.

Intended Uses: This MANalpha ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse MANalpha. This ELISA kit for research use only, not for therapeutic or test applications!

Product Categories/Family for MAN2B1 elisa kit

Signal Transduction; Metabolism; Tags & Cell Markers

NCBI and Uniprot Product Information

• NCBI GI #: 1419374
• NCBI GeneID: 4125
• Molecular Weight: 113,616 Da
• NCBI Official Full Name: alpha-mannosidase
• NCBI Official Synonym Full Names: mannosidase alpha class 2B member 1
• NCBI Official Symbol: MAN2B1
• NCBI Official Synonym Symbols: MANB; LAMAN
• NCBI Protein Information: lysosomal alpha-mannosidase
• UniProt Protein Name: Lysosomal alpha-mannosidase
• UniProt Gene Name: MAN2B1
• UniProt Synonym Gene Names: LAMAN; MANB; Laman