Rabbit anti-Human SLC29A3 Polyclonal Antibody | anti-SLC29A3 antibody

SLC29A3, NT (SLC29A3, ENT3, Equilibrative nucleoside transporter 3, Solute carrier family 29 member 3) (Azide free) (HRP)

Cat. Num. AAA6348312

• Gene Names: SLC29A3; ENT3; HJCD; PHID; HCLAP
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SLC29A3; Polyclonal Antibody; NT (SLC29A3; ENT3; Equilibrative nucleoside transporter 3; Solute carrier family 29 member 3) (Azide free) (HRP); Solute carrier family 29 member 3; anti-SLC29A3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-SLC29A3 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: SLC29A3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 28-59 amino acids from the N-terminal region of human SLC29A3.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SLC29A3 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the SLC29A3 antibody detected the SLC29A3 protein (arrow).)

Immunohistochemistry (IHC)

(SLC29A3 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SLC29A3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-SLC29A3 antibody

This gene encodes a nucleoside transporter. The encoded protein plays a role in cellular uptake of nucleosides, nucleobases, and their related analogs. Mutations in this gene have been associated with H syndrome, which is characterized by cutaneous hyperpigmentation and hypertrichosis, hepatosplenomegaly, heart anomalies, and hypogonadism. A related disorder, PHID (pigmented hypertrichosis with insulin-dependent diabetes mellitus), has also been associated with mutations at this locus. Alternatively spliced transcript variants have been described.

Product Categories/Family for anti-SLC29A3 antibody

Antibodies; Transport Proteins

References

Gass, N., et al. J Affect Disord 126 (1-2), 134-139 (2010): Kang, N., et al. J. Biol. Chem. 285(36):28343-28352(2010). Li, X., et al. Zhongguo Fei Ai Za Zhi 13(5):458-463(2010). Priya, T.P., et al. Br. J. Dermatol. 162(5):1132-1134(2010). Cliffe, S.T., et al. Hum. Mol. Genet. 18(12):2257-2265(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 148596922
• NCBI GeneID: 55315
• NCBI Accession #: NP_060814.4
• NCBI GenBank Nucleotide #: NM_018344.5
• UniProt Accession #: Q9BZD2; Q0VAM9; Q5T465; Q7RTT8; Q8IVZ0; Q9BWI2; Q9NUS9; B2RB50; B4E2Z9; B7ZA37
• Molecular Weight: 35,502 Da
• NCBI Official Full Name: equilibrative nucleoside transporter 3 isoform a
• NCBI Official Synonym Full Names: solute carrier family 29 member 3
• NCBI Official Symbol: SLC29A3
• NCBI Official Synonym Symbols: ENT3; HJCD; PHID; HCLAP
• NCBI Protein Information: equilibrative nucleoside transporter 3
• UniProt Protein Name: Equilibrative nucleoside transporter 3
• Protein Family: Equilibrative nucleoside transporter
• UniProt Gene Name: SLC29A3
• UniProt Synonym Gene Names: ENT3; hENT3
• UniProt Entry Name: S29A3_HUMAN

NCBI Description

This gene encodes a nucleoside transporter. The encoded protein plays a role in cellular uptake of nucleosides, nucleobases, and their related analogs. Mutations in this gene have been associated with H syndrome, which is characterized by cutaneous hyperpigmentation and hypertrichosis, hepatosplenomegaly, heart anomalies, and hypogonadism. A related disorder, PHID (pigmented hypertrichosis with insulin-dependent diabetes mellitus), has also been associated with mutations at this locus. Alternatively spliced transcript variants have been described.[provided by RefSeq, Mar 2010]

Uniprot Description

SLC29A3: Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). Mediates transport of adenine, adenosine and uridine, as well as several nucleoside analog drugs, such as anticancer and antiviral agents, including cladribine, cordycepin, tubercidin and AZT. Does not transport hypoxanthine. Defects in SLC29A3 are the cause of histiocytosis- lymphadenopathy plus syndrome (HLAS). A syndrome characterized by the combination of features from 2 or more of four histiocytic disorders, originally thought to be distinct: Faisalabad histiocytosis (FHC), sinus histiocytosis with massive lymphadenopathy (SHML), H syndrome, and pigmented hypertrichosis with insulin-dependent diabetes mellitus syndrome (PHID). FHC features include joint deformities, sensorineural hearing loss, and subsequent development of generalized lymphadenopathy and swellings in the eyelids that contain histiocytes. SHML causes lymph node enlargement in children frequently accompanied by fever, leukocytosis, elevated erythrocyte sedimentation rate, and polyclonal hypergammaglobulinemia. H syndrome is characterized by cutaneous hyperpigmentation and hypertrichosis, hepatosplenomegaly, heart anomalies, and hypogonadism; hearing loss is found in about half of patients. PHID is characterized by predominantly antibody-negative insulin-dependent diabetes mellitus associated with pigmented hypertrichosis and variable occurrence of other features of H syndrome. Belongs to the SLC29A/ENT transporter (TC 2.A.57) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter; Transporter, SLC family; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 10q22.1

Cellular Component: late endosome membrane; lysosomal membrane; integral to membrane

Molecular Function: nucleoside transmembrane transporter activity

Biological Process: transmembrane transport

Disease: Histiocytosis-lymphadenopathy Plus Syndrome