Rabbit anti-Human SLC22A1 Polyclonal Antibody | anti-SLC22A1 antibody

SLC22A1, CT (SLC22A1, OCT1, Solute carrier family 22 member 1, Organic cation transporter 1) (MaxLight 750)

Cat. Num. AAA6348152

• Gene Names: SLC22A1; OCT1; HOCT1; oct1_cds
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SLC22A1; Polyclonal Antibody; CT (SLC22A1; OCT1; Solute carrier family 22 member 1; Organic cation transporter 1) (MaxLight 750); Organic cation transporter 1; anti-SLC22A1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.

• Applicable Applications for anti-SLC22A1 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: SLC22A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 510-539 amino acids from the C-terminal region of human SLC22A1.
• Conjugate: MaxLight750
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Related Product Information for anti-SLC22A1 antibody

Polyspecific organic cation transporters in the liver, kidney, intestine, and other organs are critical for elimination of many endogenous small organic cations as well as a wide array of drugs and environmental toxins. This gene is one of three similar cation transporter genes located in a cluster on chromosome 6. The encoded protein contains twelve putative transmembrane domains and is a plasma integral membrane protein. Two transcript variants encoding two different isoforms have been found for this gene, but only the longer variant encodes a functional transporter. [provided by RefSeq].

Product Categories/Family for anti-SLC22A1 antibody

Antibodies; Transport Proteins

References

Takahashi, N., et al. J. Hum. Genet. 55(11):731-737(2010). Jablonski, K.A., et al. Diabetes 59(10):2672-2681(2010). Hu, M., et al. Pharmacogenet. Genomics 20(10):634-637(2010). Gambineri, A., et al. J. Clin. Endocrinol. Metab. 95 (10), E204-E208 (2010): Becker, M.L., et al. Neurogenetics (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 4506999
• NCBI GeneID: 6580
• NCBI Accession #: NP_003048.1; NP_694857.1
• NCBI GenBank Nucleotide #: NM_003057.2
• UniProt Accession #: O15245; O15395; Q9NQD4; A6NFF3; A8K1H2; C9JSU6
• Molecular Weight: 39,224 Da
• NCBI Official Full Name: solute carrier family 22 member 1 isoform a
• NCBI Official Synonym Full Names: solute carrier family 22 (organic cation transporter), member 1
• NCBI Official Symbol: SLC22A1
• NCBI Official Synonym Symbols: OCT1; HOCT1; oct1_cds
• NCBI Protein Information: solute carrier family 22 member 1; organic cation transporter 1
• UniProt Protein Name: Solute carrier family 22 member 1
• Protein Family: Solute carrier family
• UniProt Gene Name: SLC22A1
• UniProt Synonym Gene Names: OCT1; hOCT1
• UniProt Entry Name: S22A1_HUMAN

NCBI Description

Polyspecific organic cation transporters in the liver, kidney, intestine, and other organs are critical for elimination of many endogenous small organic cations as well as a wide array of drugs and environmental toxins. This gene is one of three similar cation transporter genes located in a cluster on chromosome 6. The encoded protein contains twelve putative transmembrane domains and is a plasma integral membrane protein. Two transcript variants encoding two different isoforms have been found for this gene, but only the longer variant encodes a functional transporter. [provided by RefSeq, Jul 2008]

Uniprot Description

SLC22A1: Translocates a broad array of organic cations with various structures and molecular weights including the model compounds 1-methyl-4-phenylpyridinium (MPP), tetraethylammonium (TEA), N-1-methylnicotinamide (NMN), 4-(4-(dimethylamino)styryl)- N-methylpyridinium (ASP), the endogenous compounds choline, guanidine, histamine, epinephrine, adrenaline, noradrenaline and dopamine, and the drugs quinine, and metformin. The transport of organic cations is inhibited by a broad array of compounds like tetramethylammonium (TMA), cocaine, lidocaine, NMDA receptor antagonists, atropine, prazosin, cimetidine, TEA and NMN, guanidine, cimetidine, choline, procainamide, quinine, tetrabutylammonium, and tetrapentylammonium. Translocates organic cations in an electrogenic and pH-independent manner. Translocates organic cations across the plasma membrane in both directions. Transports the polyamines spermine and spermidine. Transports pramipexole across the basolateral membrane of the proximal tubular epithelial cells. The choline transport is activated by MMTS. Regulated by various intracellular signaling pathways including inhibition by protein kinase A activation, and endogenously activation by the calmodulin complex, the calmodulin- dependent kinase II and LCK tyrosine kinase. Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter, SLC family; Transporter; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 6q25.3

Cellular Component: membrane; basolateral plasma membrane; integral to plasma membrane; plasma membrane

Molecular Function: protein binding; protein homodimerization activity; acetylcholine transmembrane transporter activity; norepinephrine transmembrane transporter activity; organic cation transmembrane transporter activity; secondary active organic cation transmembrane transporter activity; dopamine transmembrane transporter activity; quaternary ammonium group transmembrane transporter activity

Biological Process: dopamine transport; epinephrine transport; norepinephrine transport; organic cation transport; quaternary ammonium group transport; neurotransmitter transport; multidrug transport; establishment and/or maintenance of transmembrane electrochemical gradient; transmembrane transport; protein homooligomerization