Immunohistochemistry (IHC)
(Anti-SLC10A1 antibody, MBS175267, IHC(P)IHC(P): Human Liver Cancer Tissue )
Rabbit SLC10A1 Polyclonal Antibody | anti-SLC10A1 antibody
Anti-SLC10A1 antibody
Predict: Mouse
Predict: Mouse
Concentration: 0.1-0.5ug/ml
Tested Species: Rat
Predicted Species: Hu, Ms
Antigen Retrieval: n/a
Immunohistochemistry (IHC) Paraffin-embedded Section:
Concentration: 0.5-1ug/ml
Tested Species: Hu, Rat
Predicted Species: Ms
Antigen Retrieval: By Heat
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
other applications have not been tested
Optimal dilutions should be determined by end users.
Immunohistochemistry (IHC)
(Anti-SLC10A1 antibody, MBS175267, IHC(P)IHC(P): Human Liver Cancer Tissue )
Immunohistochemistry (IHC)
(Anti-SLC10A1 antibody, MBS175267, IHC(P)IHC(P): Human Liver Cancer Tissue )
Western Blot (WB)
(Anti-SLC10A1 antibody, MBS175267, Western blottingAll lanes: Anti SLC10A1 (MBS175267) at 0.5ug/mlLane 1: Rat Liver Tissue Lysate at 50ugLane 2: Mouse Liver Tissue Lysate at 50ugPredicted bind size: 38-56KDObserved bind size: 49KD )
Western Blot (WB)
(Anti-SLC10A1 antibody, MBS175267, Western blottingAll lanes: Anti SLC10A1 (MBS175267) at 0.5ug/mlLane 1: Rat Liver Tissue Lysate at 50ugLane 2: Mouse Liver Tissue Lysate at 50ugPredicted bind size: 38-56KDObserved bind size: 49KD )
Background: SLC10A1(SOLUTE CARRIER FAMILY 10(SODIUM/BILE ACID COTRANSPORTER FAMILY), MEMBER 1),also called NTCP or NTCP1, is found in the basolateral membranes of hepatocytes, which is also involved in the reabsorption of bile acids. The SLC10A1 gene is mapped on 14q24.2. The SLC10A1 gene contains 5 exons and spans about 23 kb. Like other sodium/bile acid transporters, SLC10A1 contains an exoplasmic N terminus, an odd number of transmembrane regions, and a cytoplasmic C terminus. Two amphipathic sequences are critical for intramolecular interactions and for proper trafficking of SLC10A1 to the plasma membrane. Injection of in vitro transcribed NTCP cRNA into Xenopus oocytes resulted in the expression of Na(+)-dependent taurocholate uptake. NTCP-mediated taurocholate uptake in oocytes was inhibited by all major bile acid derivatives and by bromosulfophthalein.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the sodium/bile acid cotransporter family, which are integral membrane glycoproteins that participate in the enterohepatic circulation of bile acids. Two homologous transporters are involved in the reabsorption of bile acids; the ileal sodium/bile acid cotransporter with an apical cell localization that absorbs bile acids from the intestinal lumen, bile duct and kidney, and the liver-specific sodium/bile acid cotransporter, represented by this protein, that is found in the basolateral membranes of hepatocytes. Bile acids are the catabolic product of cholesterol metabolism, hence this protein is important for cholesterol homeostasis. [provided by RefSeq, Oct 2011]
Uniprot Description
NTCP: The hepatic sodium/bile acid uptake system exhibits broad substrate specificity and transports various non-bile acid organic compounds as well. It is strictly dependent on the extracellular presence of sodium. Belongs to the bile acid:sodium symporter (BASS) (TC 2.A.28) family.
Protein type: Membrane protein, multi-pass; Membrane protein, integral; Transporter
Chromosomal Location of Human Ortholog: 14q24.1
Cellular Component: integral to plasma membrane; basolateral plasma membrane; plasma membrane
Molecular Function: bile acid:sodium symporter activity
Biological Process: bile acid and bile salt transport; bile acid metabolic process; transport; sodium ion transport; transmembrane transport
Research Articles on SLC10A1
Similar Products
Product Notes
The SLC10A1 slc10a1 (Catalog #AAA175267) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SLC10A1 antibody reacts with Human, Rat Predict: Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SLC10A1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. Western Blot (WB): Concentration: 0.1-0.5ug/ml Tested Species: Rat Predicted Species: Hu, Ms Antigen Retrieval: Immunohistochemistry (IHC) Paraffin-embedded Section: Concentration: 0.5-1ug/ml Tested Species: Hu, Rat Predicted Species: Ms Antigen Retrieval: By Heat Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. other applications have not been tested Optimal dilutions should be determined by end users. Researchers should empirically determine the suitability of the SLC10A1 slc10a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SLC10A1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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