Rabbit anti-Human SLC10A1 Polyclonal Antibody | anti-SLC10A1 antibody

SLC10A1, CT (SLC10A1, NTCP, Sodium/bile acid cotransporter, Cell growth-inhibiting gene 29 protein, Na(+)/bile acid cotransporter, Na(+)/taurocholate transport protein, Sodium/taurocholate cotransporting polypeptide, Solute carrier family 10 member 1) (Az

Cat. Num. AAA6347982

• Gene Names: SLC10A1; NTCP
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: SLC10A1; Polyclonal Antibody; CT (SLC10A1; NTCP; Sodium/bile acid cotransporter; Cell growth-inhibiting gene 29 protein; Na(+)/bile acid cotransporter; Na(+)/taurocholate transport protein; Sodium/taurocholate cotransporting polypeptide; Solute carrier family 10 member 1) (Az; Solute carrier family 10 member 1; anti-SLC10A1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SLC10A1.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-SLC10A1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 316-343 in the C-terminal region of human SLC10A1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot using MBS647059 at 1:1000 dilution Lane 1: HT-1080 whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: K562 whole cell lysate Lane 4: Caco2 whole cell lysate Lysates/proteins at 20ug per lane. Secondary IgG (HRP) Goat Anti-Rabbit Predicted band size : 38kD Blocking/Dilution buffer: 5% NFDM/TBST.)

Testing Data

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Related Product Information for anti-SLC10A1 antibody

Sodium/bile acid cotransporters are integral membrane glycoproteins that participate in the enterohepatic circulation of bile acids. Two homologous transporters are involved in the reabsorption of bile acids, one absorbing from the intestinal lumen, the bile duct, and the kidney with an apical localization (SLC10A2; MIM 601295), and the other being found in the basolateral membranes of hepatocytes (SLC10A1).

Product Categories/Family for anti-SLC10A1 antibody

Antibodies; Transport Proteins

References

Ho, R.H., et al. J. Biol. Chem. 279(8):7213-7222(2004). Trauner, M., et al. Physiol. Rev. 83(2):633-671(2003). Hallen, S., et al. Biochemistry 41(23):7253-7266(2002). Shiao, T., et al. Genomics 69(2):203-213(2000). Hagenbuch, B., et al. J. Clin. Invest. 93(3):1326-1331(1994).

NCBI and Uniprot Product Information

• NCBI GI #: 4506971
• NCBI GeneID: 6554
• NCBI Accession #: NP_003040.1
• NCBI GenBank Nucleotide #: NM_003049.3
• UniProt Accession #: Q14973; Q2TU29; B9EGB6
• Molecular Weight: 38,119 Da
• NCBI Official Full Name: sodium/bile acid cotransporter
• NCBI Official Synonym Full Names: solute carrier family 10 member 1
• NCBI Official Symbol: SLC10A1
• NCBI Official Synonym Symbols: NTCP
• NCBI Protein Information: sodium/bile acid cotransporter
• UniProt Protein Name: Sodium/bile acid cotransporter
• Protein Family: Sodium/bile acid cotransporter
• UniProt Gene Name: SLC10A1
• UniProt Synonym Gene Names: NTCP
• UniProt Entry Name: NTCP_HUMAN

NCBI Description

The protein encoded by this gene belongs to the sodium/bile acid cotransporter family, which are integral membrane glycoproteins that participate in the enterohepatic circulation of bile acids. Two homologous transporters are involved in the reabsorption of bile acids; the ileal sodium/bile acid cotransporter with an apical cell localization that absorbs bile acids from the intestinal lumen, bile duct and kidney, and the liver-specific sodium/bile acid cotransporter, represented by this protein, that is found in the basolateral membranes of hepatocytes. Bile acids are the catabolic product of cholesterol metabolism, hence this protein is important for cholesterol homeostasis. [provided by RefSeq, Oct 2011]

Uniprot Description

NTCP: The hepatic sodium/bile acid uptake system exhibits broad substrate specificity and transports various non-bile acid organic compounds as well. It is strictly dependent on the extracellular presence of sodium. Belongs to the bile acid:sodium symporter (BASS) (TC 2.A.28) family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Transporter

Chromosomal Location of Human Ortholog: 14q24.1

Cellular Component: integral to plasma membrane; basolateral plasma membrane; plasma membrane

Molecular Function: bile acid:sodium symporter activity

Biological Process: bile acid and bile salt transport; bile acid metabolic process; transport; sodium ion transport; transmembrane transport