Rabbit anti-Human SKI Polyclonal Antibody | anti-SKI antibody

Rabbit Polyclonal to Human SKI

Cat. Num. AAA240370

• Gene Names: SKI; SGS; SKV
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: SKI; Polyclonal Antibody; Rabbit Polyclonal to Human SKI; Anti-SKI Antibody (N-Terminus) IHC-plus; SKV; Proto-oncogene c-Ski; Ski oncogene; Ski oncoprotein; SGS; Human SKI; anti-SKI antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: 14 amino acid peptide from near the amino terminus of human Ski
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.02% sodium azide.
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 728

• Applicable Applications for anti-SKI antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (2.5 ug/ml), WB; Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...
• Target Species: Human
• Immunogen Description: Synthetic peptide from human SKI.
• Immunogen Type: Synthetic peptide
• Immunogen: SKI antibody was raised against synthetic peptide from human SKI.
• Antigen Modification: N-Terminus
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.

Immunohistochemistry (IHC)

(Anti-SKI antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-SKI antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of Ski in Human Kidney cells with Ski antibody at 20 ug/ml.)

Western Blot (WB)

(Western blot of Ski in human kidney tissue lysate with Ski antibody at (A) 1 and (B) 2 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 4506967
• NCBI GeneID: 6497
• NCBI Accession #: NP_003027.1
• NCBI GenBank Nucleotide #: NM_003036.3
• UniProt Accession #: P12755; Q5SYT7
• Molecular Weight: 80,005 Da
• NCBI Official Full Name: ski oncogene
• NCBI Official Synonym Full Names: SKI proto-oncogene
• NCBI Official Symbol: SKI
• NCBI Official Synonym Symbols: SGS; SKV
• NCBI Protein Information: ski oncogene; ski oncoprotein; proto-oncogene c-Ski; v-ski avian sarcoma viral oncogene homolog
• UniProt Protein Name: Ski oncogene
• Protein Family: Ski oncogene
• UniProt Gene Name: SKI
• UniProt Entry Name: SKI_HUMAN

NCBI Description

This gene encodes the nuclear protooncogene protein homolog of avian sarcoma viral (v-ski) oncogene. It functions as a repressor of TGF-beta signaling, and may play a role in neural tube development and muscle differentiation. [provided by RefSeq, Oct 2009]

Uniprot Description

SKI: May play a role in terminal differentiation of skeletal muscle cells but not in the determination of cells to the myogenic lineage. Functions as a repressor of TGF-beta signaling. Belongs to the SKI family.

Protein type: Cell cycle regulation; Ubiquitin conjugating system; Oncoprotein; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 1p36.33

Cellular Component: nucleoplasm; nuclear body; centrosome; transcription factor complex; PML body; protein complex; transcriptional repressor complex; cytoplasm; nucleus

Molecular Function: protein domain specific binding; histone deacetylase inhibitor activity; protein binding; zinc ion binding; ubiquitin protein ligase binding; chromatin binding; SMAD binding; protein kinase binding; transcription corepressor activity

Biological Process: somatic stem cell maintenance; cell motility involved in cell locomotion; skeletal muscle fiber development; palate development; negative regulation of transcription from RNA polymerase II promoter; negative regulation of BMP signaling pathway; BMP signaling pathway; nose morphogenesis; regulation of apoptosis; negative regulation of cell proliferation; transforming growth factor beta receptor signaling pathway; negative regulation of osteoblast differentiation; positive regulation of Wnt receptor signaling pathway; embryonic limb morphogenesis; positive regulation of DNA binding; transcription initiation from RNA polymerase II promoter; camera-type eye development; negative regulation of histone deacetylation; transcription, DNA-dependent; olfactory bulb development; myotube differentiation; myelination in the peripheral nervous system; negative regulation of activin receptor signaling pathway; cell proliferation; camera-type eye morphogenesis; retina development in camera-type eye; negative regulation of fibroblast proliferation; neural tube closure; gene expression; lens morphogenesis in camera-type eye; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transforming growth factor beta receptor signaling pathway; anterior/posterior axis specification

Disease: Shprintzen-goldberg Craniosynostosis Syndrome