Rabbit anti-Human, Mouse Sirtuin 7 Polyclonal Antibody | anti-SIRT7 antibody

Sirtuin 7 (NAD-dependent Protein Deacetylase Sirtuin-7, SIRT7, Regulatory Protein SIR2 Homolog 7, SIR2-like Protein 7, SIR2L7) (FITC)

Cat. Num. AAA6394215

• Gene Names: SIRT7; SIR2L7
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Sirtuin 7; Polyclonal Antibody; Sirtuin 7 (NAD-dependent Protein Deacetylase Sirtuin-7; SIRT7; Regulatory Protein SIR2 Homolog 7; SIR2-like Protein 7; SIR2L7) (FITC); EC=3.5.1.-; anti-SIRT7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SIRT7. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-SIRT7 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human SIRT7, aa1-400 (NP_057622.1).
• Immunogen Sequence: MAAGGLSRSERKAAERVRRLREEQQRERLRQVSRILRKAAAERSAEEGRLLAESADLVTELQGRSRRREGLKRRQEEVCDDPEELRGKVRELASAVRNAKYLVVYTGAGISTAASIPDYRGPNGVWTLLQKGRSVSAADLSEAEPTLTHMSITRLHEQKLVQHVVSQNCDGLHLRSGLPRTAISELHGNMYIEVCTSCVPNREYVRVFDVTERTALHRHQTGRTCHKCGTQLRDTIVHFGERGTLGQPLNWEAATEAASRADTILCLGSSLKVLKKYPRLWCMTKPPSRRPKLYIVNLQWTPKDDWAALKLHGKCDDVMRLLMAELGLEIPAYSRWQDPIFSLATPLRAGEEGSHSRKSLCRSREEAPPGDRGAPLSSAPILGGWFGRGCTKRTKRKKVT
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SIRT7 rabbit polyclonal antibody. Western Blot analysis of SIRT7 expression in human liver.)

Western Blot (WB)

(SIRT7 rabbit polyclonal antibody. Western Blot analysis of SIRT7 expression in mouse liver.)

Western Blot (WB)

(Western Blot analysis of SIRT7 expression in transfected 293T cell line by SIRT7 polyclonal antibody. Lane 1: SIRT7 transfected lysate (44.9kD). Lane 2: Non-transfected lysate.)

Product Categories/Family for anti-SIRT7 antibody

Antibodies; Sirtuins

NCBI and Uniprot Product Information

• NCBI GI #: 7706712
• NCBI GeneID: 51547
• NCBI Accession #: NP_057622.1
• NCBI GenBank Nucleotide #: NM_016538.1
• UniProt Accession #: Q9NRC8; Q3MIK4; Q9NSZ6; Q9NUS6; A8K2K0; B3KSU8
• Molecular Weight: 400
• NCBI Official Full Name: NAD-dependent protein deacetylase sirtuin-7
• NCBI Official Synonym Full Names: sirtuin 7
• NCBI Official Symbol: SIRT7
• NCBI Official Synonym Symbols: SIR2L7
• NCBI Protein Information: NAD-dependent protein deacetylase sirtuin-7; sirtuin type 7; SIR2-like protein 7; sir2-related protein type 7; regulatory protein SIR2 homolog 7; NAD-dependent deacetylase sirtuin-7; silent mating type information regulation 2, S.cerevisiae, homolog 7
• UniProt Protein Name: NAD-dependent protein deacetylase sirtuin-7
• Protein Family: NAD-dependent protein deacetylase
• UniProt Gene Name: SIRT7
• UniProt Synonym Gene Names: SIR2L7
• UniProt Entry Name: SIR7_HUMAN

Uniprot Description

SIRT7: NAD-dependent protein deacetylase that specifically mediates deacetylation of histone H3 at 'Lys-18' (H3K18Ac). In contrast to other histone deacetylases, displays selectivity for a single histone mark, H3K18Ac, directly linked to control of gene expression. H3K18Ac is mainly present around the transcription start site of genes and has been linked to activation of nuclear hormone receptors. SIRT7 thereby acts as a transcription repressor. Moreover, H3K18 hypoacetylation has been reported as a marker of malignancy in various cancers and seems to maintain the transformed phenotype of cancer cells. These data suggest that SIRT7 may play a key role in oncogenic transformation by suppresses expression of tumor suppressor genes by locus-specific deacetylation of H3K18Ac at promoter regions. Also required to restore the transcription of ribosomal RNA (rRNA) at the exit from mitosis: promotes the association of RNA polymerase I with the rDNA promoter region and coding region. Stimulates transcription activity of the RNA polymerase I complex. May also deacetylate p53/TP53 and promotes cell survival, however such data need additional confirmation. Belongs to the sirtuin family. Class IV subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.5.1.-; Deacetylase; Nucleolus

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: nucleolus organizer region; cytoplasm; nucleolus; nucleus

Molecular Function: protein binding; metal ion binding; chromatin binding

Biological Process: activation of transcription on exit from mitosis; negative regulation of transcription from RNA polymerase II promoter; rRNA transcription