Western Blot (WB)
(Western Blot: Sample: Lane1: Mouse Liver Tissue; Lane2: Human Hela Cells; Lane3: Human PC-3 Cells.)
Rabbit anti-Mouse Sirtuin 4 (SIRT4) Polyclonal Antibody | anti-SIRT4 antibody
Polyclonal Antibody to Sirtuin 4 (SIRT4)
Gene Names
Sirt4; 4930596O17Rik
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Sirtuin 4 (SIRT4); Polyclonal Antibody; Polyclonal Antibody to Sirtuin 4 (SIRT4); anti-SIRT4 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against SIRT4. It has been selected for its ability to recognize SIRT4 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with a His-tag and its sequence is listed below.
MGHHHHHHSGSEF-SGLFV PPSPPLDPEK IKELQRFISL SKKLLVMTGA GISTESGIPD YRSEKVGLYA RTDRRPIQHI DFVRSAPVRQ RYWARNFVGW PQFSSHQPNP AHWALSNWER LGKLHWLVTQ NVDALHSKAG SQRLTELHGC MHRVLCLNCG EQTARRVLQE RFQALNPSWS AEAQGVAPDG DVFLTEEQVR SFQVPCCDRC GGPLKPDVVF FGDTVNPDKV DFVHRRVKEA DSLLVVGSSL QVYSGYRFIL TAREQKLPIA ILNIGPTRSD DLACLKLDSR CGELLPLIDP RRQHSDVQRL EMNFPLSSAA QDP
MGHHHHHHSGSEF-SGLFV PPSPPLDPEK IKELQRFISL SKKLLVMTGA GISTESGIPD YRSEKVGLYA RTDRRPIQHI DFVRSAPVRQ RYWARNFVGW PQFSSHQPNP AHWALSNWER LGKLHWLVTQ NVDALHSKAG SQRLTELHGC MHRVLCLNCG EQTARRVLQE RFQALNPSWS AEAQGVAPDG DVFLTEEQVR SFQVPCCDRC GGPLKPDVVF FGDTVNPDKV DFVHRRVKEA DSLLVVGSSL QVYSGYRFIL TAREQKLPIA ILNIGPTRSD DLACLKLDSR CGELLPLIDP RRQHSDVQRL EMNFPLSSAA QDP
Applicable Applications for anti-SIRT4 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant SIRT4 (Ser26~Pro333) expressed in E.coli.
Cross Reactivity
Human, Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2075803
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Lane1: Mouse Liver Tissue; Lane2: Human Hela Cells; Lane3: Human PC-3 Cells.)
Western Blot (WB)
(Western Blot: Sample: Lane1: Mouse Liver Tissue; Lane2: Human Hela Cells; Lane3: Human PC-3 Cells.)
Western Blot (WB)
(Western Blot: Sample: Recombinant SIRT4, Mouse.)
Western Blot (WB)
(Western Blot: Sample: Recombinant SIRT4, Mouse.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded liver tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded liver tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
37,525 Da
NCBI Official Full Name
NAD-dependent protein lipoamidase sirtuin-4, mitochondrial
NCBI Official Synonym Full Names
sirtuin 4
NCBI Official Symbol
Sirt4
NCBI Official Synonym Symbols
4930596O17Rik
NCBI Protein Information
NAD-dependent protein lipoamidase sirtuin-4, mitochondrial
UniProt Protein Name
NAD-dependent protein lipoamidase sirtuin-4, mitochondrial
Protein Family
UniProt Gene Name
Sirt4
UniProt Synonym Gene Names
Sir2l4
Uniprot Description
SIRT4: NAD-dependent protein ADP-ribosyl transferase. Catalyzes the transfer of ADP-ribosyl groups onto target proteins, including mitochondrial GLUD1. Inhibits GLUD1 enzyme activity. Down- regulates insulin secretion. Has no detectable protein deacetylase activity. Belongs to the sirtuin family. Class II subfamily.
Protein type: Deacetylase; EC 2.4.2.-; EC 3.5.1.-; Mitochondrial; Tumor suppressor
Chromosomal Location of Human Ortholog: 5|5 F
Cellular Component: mitochondrial inner membrane; mitochondrial matrix; mitochondrion
Molecular Function: biotinidase activity; hydrolase activity; metal ion binding; NAD+ ADP-ribosyltransferase activity; protein binding; transferase activity
Biological Process: cellular response to DNA damage stimulus; glutamine metabolic process; negative regulation of fatty acid oxidation; negative regulation of insulin secretion; positive regulation of lipid biosynthetic process; protein ADP-ribosylation; regulation of glutamine family amino acid metabolic process
Research Articles on SIRT4
Similar Products
Product Notes
The SIRT4 sirt4 (Catalog #AAA2006109) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Sirtuin 4 (SIRT4) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Sirtuin 4 (SIRT4) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the SIRT4 sirt4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with a His-tag and its sequence is listed below. MGHHHHHHSG SEF-SGLFV PPSPPLDPEK IKELQRFISL SKKLLVMTGA GISTESGIPD YRSEKVGLYA RTDRRPIQHI DFVRSAPVRQ RYWARNFVGW PQFSSHQPNP AHWALSNWER LGKLHWLVTQ NVDALHSKAG SQRLTELHGC MHRVLCLNCG EQTARRVLQE RFQALNPSWS AEAQGVAPDG DVFLTEEQVR SFQVPCCDRC GGPLKPDVVF FGDTVNPDKV DFVHRRVKEA DSLLVVGSSL QVYSGYRFIL TAREQKLPIA ILNIGPTRSD DLACLKLDSR CGELLPLIDP RRQHSDVQRL EMNFPLSSAA QDP. It is sometimes possible for the material contained within the vial of "Sirtuin 4 (SIRT4), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
