Rabbit anti-Human, Mouse SIK1 / MSK Polyclonal Antibody | anti-SIK1 antibody

Rabbit Polyclonal to Human SIK1 / MSK

Cat. Num. AAA248499

• Gene Names: SIK1; MSK; SIK; SNF1LK
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Ammonium sulfate precipitation
• Synonyms: SIK1 / MSK; Polyclonal Antibody; Rabbit Polyclonal to Human SIK1 / MSK; Anti-SIK1 / MSK Antibody (C-Terminus) IHC-plus; SIK1; KID2; Salt-inducible kinase; Salt-inducible kinase 1; Salt-inducible protein kinase; SIK; MSK; SIK-1; SNF1-like kinase; Myocardial SNF1-like kinase; QIK; Qin-induced kinase; SNF1LK; Human SIK1; anti-SIK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Purity/Purification: Ammonium sulfate precipitation
• Form/Format: PBS, 0.09% sodium azide
• Sequence Length: 783

• Applicable Applications for anti-SIK1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (1:100), WB (1:1000)
• Target Species: Human
• Immunogen Description: KLH conjugated synthetic peptide between 567-597 amino acids from the C-terminal region of human SNF1LK.
• Immunogen: SIK1 / MSK antibody was raised against kLH conjugated synthetic peptide between 567-597 amino acids from the C-terminal region of human SNF1LK.
• Antigen Modification: C-Terminus
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Immunohistochemistry (IHC)

(Anti-SIK1 / MSK antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.)

Immunohistochemistry (IHC)

(Western blot of SNF1LK antibody to detect SNF1LK in Sk-Br-3 cell lysate.)

Western Blot (WB)

(SNF1LK Antibody (C-term) western blot analysis in mouse brain tissue lysates (35 ug/lane).This demonstrates the SNF1LK antibody detected the SNF1LK protein (arrow).)

Related Product Information for anti-SIK1 antibody

Salt-Inducible Protein Kinase belongs to the AMPK/SNF1 kinase subfamily. Caution: The symbol SIK also is used for the Src-related intestinal kinase (PTK6) on chromosome 20.

Product Categories/Family for anti-SIK1 antibody

Family: Protein Kinase; Subfamily: AMPK/SNF1

NCBI and Uniprot Product Information

• NCBI GI #: 116256471
• NCBI GeneID: 150094
• NCBI Accession #: NP_775490.2
• NCBI GenBank Nucleotide #: NM_173354.3
• UniProt Accession #: P57059; Q5R2V5; Q6ZNL8; Q86YJ2; A6NC84
• Molecular Weight: 84,902 Da
• NCBI Official Full Name: serine/threonine-protein kinase SIK1
• NCBI Official Synonym Full Names: salt-inducible kinase 1
• NCBI Official Symbol: SIK1
• NCBI Official Synonym Symbols: MSK; SIK; SNF1LK
• NCBI Protein Information: serine/threonine-protein kinase SIK1; SIK-1; SNF1-like kinase; myocardial SNF1-like kinase; salt-inducible protein kinase 1; serine/threonine protein kinase; serine/threonine-protein kinase SNF1LK; serine/threonine-protein kinase SNF1-like kinase 1
• UniProt Protein Name: Serine/threonine-protein kinase SIK1
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: SIK1
• UniProt Synonym Gene Names: SIK; SNF1LK; SIK-1; Serine/threonine-protein kinase SNF1LK
• UniProt Entry Name: SIK1_HUMAN

Uniprot Description

SIK: Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, gluconeogenesis and lipogenesis regulation, muscle growth and differentiation and tumor suppression. Phosphorylates HDAC4, HDAC5, PPME1, SREBF1, TORC1/CRTC1 and TORC2/CRTC2. Acts as a tumor suppressor and plays a key role in p53/TP53-dependent anoikis, a type of apoptosis triggered by cell detachment: required for phosphorylation of p53/TP53 in response to loss of adhesion and is able to suppress metastasis. Part of a sodium-sensing signaling network, probably by mediating phosphorylation of PPME1: following increases in intracellular sodium, SIK1 is activated by CaMK1 and phosphorylates PPME1 subunit of protein phosphatase 2A (PP2A), leading to dephosphorylation of sodium/potassium-transporting ATPase ATP1A1 and subsequent increase activity of ATP1A1. Acts as a regulator of muscle cells by phosphorylating and inhibiting class II histone deacetylases HDAC4 and HDAC5, leading to promote expression of MEF2 target genes in myocytes. Also required during cardiomyogenesis by regulating the exit of cardiomyoblasts from the cell cycle via down-regulation of CDKN1C/p57Kip2. Acts as a regulator of hepatic gluconeogenesis by phosphorylating and repressing the CREB-specific coactivators TORC1/CRTC1 and TORC2/CRTC2, leading to inhibit CREB activity. Also regulates hepatic lipogenesis by phosphorylating and inhibiting SREBF1. Interacts with ATP1A1. Interacts (when phosphorylated on Thr-182 and Ser-186) with YWHAZ. Activated by phosphorylation on Thr-182. Also activated by phosphorylation on Thr-322 in response to increases in intracellular sodium in parallel with elevations in intracellular calcium through the reversible sodium/calcium exchanger. Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. AMPK subfamily.

Protein type: Tumor suppressor; Kinase, protein; Protein kinase, CAMK; Motility/polarity/chemotaxis; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); CAMK group; CAMKL family; QIK subfamily

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: cytoplasm; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; cAMP response element binding protein binding; magnesium ion binding; protein kinase binding; ATP binding

Biological Process: regulation of cell differentiation; protein amino acid autophosphorylation; negative regulation of gluconeogenesis; regulation of mitotic cell cycle; rhythmic process; regulation of sodium ion transport; cell cycle; inhibition of CREB transcription factor; protein amino acid phosphorylation; cardiac muscle cell differentiation; entrainment of circadian clock by photoperiod

Disease: Epileptic Encephalopathy, Early Infantile, 30