Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Western Blot (WB) (SIGLEC8 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the SIGLEC8 antibody detected the SIGLEC8 protein (arrow).)

Rabbit anti-Human Siglec 8 Polyclonal Antibody | anti-SIGLEC8 antibody

Siglec 8, NT (Siglec 8, SAF2, Sialic acid-binding Ig-like lectin 8, CDw329, Sialoadhesin family member 2, CD329) (Biotin)

Gene Names
SIGLEC8; SAF2; SIGLEC-8; SIGLEC8L
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
Siglec 8; Polyclonal Antibody; NT (Siglec 8; SAF2; Sialic acid-binding Ig-like lectin 8; CDw329; Sialoadhesin family member 2; CD329) (Biotin); SIGLEC8; CD329; anti-SIGLEC8 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Applicable Applications for anti-SIGLEC8 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:10-50
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
SIGLEC8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 93-122 amino acids from the N-terminal region of human SIGLEC8.
Conjugate
Biotin
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SIGLEC8 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the SIGLEC8 antibody detected the SIGLEC8 protein (arrow).)

Western Blot (WB) (SIGLEC8 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the SIGLEC8 antibody detected the SIGLEC8 protein (arrow).)

Immunohistochemistry (IHC)

(SIGLEC8 Antibody (N-term) (AP14564a)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SIGLEC8 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (SIGLEC8 Antibody (N-term) (AP14564a)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SIGLEC8 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Product Categories/Family for anti-SIGLEC8 antibody
References
Gao, P.S., et al. Eur. J. Hum. Genet. 18(6):713-719(2010). Davila, S., et al. Genes Immun. 11(3):232-238(2010). Hudson, S.A., et al. J. Pharmacol. Exp. Ther. 330(2):608-612(2009). Bochner, B.S. Clin. Exp. Allergy 39(3):317-324(2009). von Gunten, S., et al. Autoimmun Rev 7(6):453-456(2008).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
46,434 Da
NCBI Official Full Name
sialic acid-binding Ig-like lectin 8
NCBI Official Synonym Full Names
sialic acid binding Ig like lectin 8
NCBI Official Symbol
SIGLEC8
NCBI Official Synonym Symbols
SAF2; SIGLEC-8; SIGLEC8L
NCBI Protein Information
sialic acid-binding Ig-like lectin 8
UniProt Protein Name
Sialic acid-binding Ig-like lectin 8
UniProt Gene Name
SIGLEC8
UniProt Synonym Gene Names
SAF2; Siglec-8; SAF-2
UniProt Entry Name
SIGL8_HUMAN

NCBI Description

Sialic acid-binding immunoglobulin (Ig)-like lectins, or SIGLECs (e.g., CD33 (MIM 159590)), are a family of type 1 transmembrane proteins each having a unique expression pattern, mostly in hemopoietic cells. SIGLEC8 is a member of the CD33-like subgroup of SIGLECs, which are localized to 19q13.3-q13.4 and have 2 conserved cytoplasmic tyrosine-based motifs: an immunoreceptor tyrosine-based inhibitory motif, or ITIM (see MIM 604964), and a motif homologous to one identified in signaling lymphocyte activation molecule (SLAM; MIM 603492) that mediates an association with SLAM-associated protein (SAP; MIM 300490) (summarized by Foussias et al., 2000 [PubMed 11095983]).[supplied by OMIM, May 2010]

Research Articles on SIGLEC8

Similar Products

Product Notes

The SIGLEC8 siglec8 (Catalog #AAA6347628) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Siglec 8, NT (Siglec 8, SAF2, Sialic acid-binding Ig-like lectin 8, CDw329, Sialoadhesin family member 2, CD329) (Biotin) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Siglec 8 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:10-50 ELISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the SIGLEC8 siglec8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Siglec 8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.