Rabbit anti-Human Siglec 5 Polyclonal Antibody | anti-SIGLEC5 antibody

Siglec 5, NT (Siglec 5, CD33L2, OBBP2, Sialic acid-binding Ig-like lectin 5, CD33 antigen-like 2, Obesity-binding protein 2, CD170) (Azide free) (HRP)

Cat. Num. AAA6347619

• Gene Names: SIGLEC5; CD170; OBBP2; CD33L2; OB-BP2; SIGLEC-5
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Siglec 5; Polyclonal Antibody; NT (Siglec 5; CD33L2; OBBP2; Sialic acid-binding Ig-like lectin 5; CD33 antigen-like 2; Obesity-binding protein 2; CD170) (Azide free) (HRP); SIGLEC5; CD170; anti-SIGLEC5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-SIGLEC5 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: SIGLEC5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 103-132 amino acids from the N-terminal region of human SIGLEC5.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SIGLEC5 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the SIGLEC5 antibody detected the SIGLEC5 protein (arrow).)

Related Product Information for anti-SIGLEC5 antibody

The sialic acid-binding immunoglobulin-like lectins (SIGLECs), such as SIGLEC5, are a subgroup of the immunoglobulin (Ig) superfamily that mediate protein-carbohydrate interactions. They specifically interact with sialic acids in glycoproteins and glycolipids, with each SIGLEC having a particular preference for both the nature of the sialic acid and its glycosidic linkage to adjacent sugars. SIGLECs have similar structures, including extracellular Ig-like domains composed of an N-terminal V-set domain followed by varying numbers of C2-set domains. It appears that all SIGLECs have an unusual arrangement of conserved cysteine residues in the V-set and adjacent C2-set domains. Most SIGLECs are expressed uniquely within the hematopoietic system (Cornish et al., 1998 [PubMed 9731071]).

Product Categories/Family for anti-SIGLEC5 antibody

Antibodies; CD Markers

References

Soto, P.C., et al. J. Immunol. 184(8):4185-4195(2010). Davila, S., et al. Genes Immun. 11(3):232-238(2010). Carlin, A.F., et al. J. Exp. Med. 206(8):1691-1699(2009). Zhuravleva, M.A., et al. J. Mol. Biol. 375(2):437-447(2008). Gunnarsson, P., et al. FASEB J. 21(14):4059-4069(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 4502659
• NCBI GeneID: 8778
• NCBI Accession #: NP_003821.1
• NCBI GenBank Nucleotide #: NM_003830.3
• UniProt Accession #: O15389
• Molecular Weight: 60,715 Da
• NCBI Official Full Name: sialic acid-binding Ig-like lectin 5
• NCBI Official Synonym Full Names: sialic acid binding Ig like lectin 5
• NCBI Official Symbol: SIGLEC5
• NCBI Official Synonym Symbols: CD170; OBBP2; CD33L2; OB-BP2; SIGLEC-5
• NCBI Protein Information: sialic acid-binding Ig-like lectin 5
• UniProt Protein Name: Sialic acid-binding Ig-like lectin 5
• Protein Family: Sialic acid-binding Ig-like lectin
• UniProt Gene Name: SIGLEC5
• UniProt Synonym Gene Names: CD33L2; OBBP2; Siglec-5; OB-BP2; OB-binding protein 2

NCBI Description

This gene encodes a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. These cell surface lectins are characterized by structural motifs in the immunoglobulin (Ig)-like domains and sialic acid recognition sites in the first Ig V set domain. The encoded protein is a member of the CD33-related subset of Siglecs and inhibits the activation of several cell types including monocytes, macrophages and neutrophils. Binding of group B Streptococcus (GBS) to the encoded protein plays a role in GBS immune evasion. [provided by RefSeq, Feb 2012]

Uniprot Description

Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds equally to alpha-2,3-linked and alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.