Rabbit anti-Human Siglec 14 Polyclonal Antibody | anti-SIGLEC14 antibody

Siglec 14, NT (Siglec 14, Sialic acid-binding Ig-like lectin 14) (PE)

Cat. Num. AAA6347614

• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Siglec 14; Polyclonal Antibody; NT (Siglec 14; Sialic acid-binding Ig-like lectin 14) (PE); SIGLEC14; Sialic acid-binding Ig-like lectin 14; anti-SIGLEC14 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-SIGLEC14 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: SIGLEC14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 59-89 amino acids from the N-terminal region of human SIGLEC14.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(SIGLEC14 Antibody (N-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the SIGLEC14 antibody detected the SIGLEC14 protein (arrow).)

Related Product Information for anti-SIGLEC14 antibody

Putative adhesion molecule. Sialic acid-binding paired receptor which may activate associated receptors.

Product Categories/Family for anti-SIGLEC14 antibody

Antibodies; Lectins

References

Yamanaka, M., et al. Glycobiology 19(8):841-846(2009). Angata, T., et al. FASEB J. 20(12):1964-1973(2006). Clark, H.F., et al. Genome Res. 13(10):2265-2270(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 148762980
• NCBI GeneID: 100049587
• NCBI Accession #: NP_001092082.1
• NCBI GenBank Nucleotide #: NM_001098612.1
• UniProt Accession #: Q08ET2; Q6UXG0
• Molecular Weight: 43,970 Da
• NCBI Official Full Name: sialic acid-binding Ig-like lectin 14
• NCBI Official Synonym Full Names: sialic acid binding Ig-like lectin 14
• NCBI Official Symbol: SIGLEC14
• NCBI Protein Information: sialic acid-binding Ig-like lectin 14; siglec-14
• UniProt Protein Name: Sialic acid-binding Ig-like lectin 14
• Protein Family: Sialic acid-binding Ig-like lectin
• UniProt Gene Name: SIGLEC14
• UniProt Synonym Gene Names: Siglec-14
• UniProt Entry Name: SIG14_HUMAN

Uniprot Description

Function: Putative adhesion molecule. Sialic acid-binding paired receptor which may activate associated receptors. Ref.1

Subunit structure: Interacts with TYROBP. Ref.1

Subcellular location: Cell membrane; Single-pass type I membrane protein Ref.1.

Tissue specificity: Mainly expressed in hematopoietic tissues including bone marrow, spleen and fetal liver. Also detected in lung and testis. Ref.1

Sequence similarities: Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.Contains 2 Ig-like C2-type (immunoglobulin-like) domains.Contains 1 Ig-like V-type (immunoglobulin-like) domain.