Rabbit SHP2 Polyclonal Antibody | anti-PTPN11 antibody

SHP2 Antibody

Cat. Num. AAA150386

• Gene Names: PTPN11; CFC; NS1; SHP2; BPTP3; PTP2C; PTP-1D; SH-PTP2; SH-PTP3
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: SHP2 Antibody is affinity chromatography purified via peptide column.
• Synonyms: SHP2; Polyclonal Antibody; SHP2 Antibody; CFC; NS1; BPTP3; PTP2C; PTP-1D; SH-PTP2; SH-PTP3; SHPTP2; Tyrosine-protein phosphatase non-receptor type 11; Protein-tyrosine phosphatase 1D; protein tyrosine phosphatase; non-receptor type 11; anti-PTPN11 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: SHP2 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 593

• Applicable Applications for anti-PTPN11 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: SHP2 antibody can be used for the detection of SHP2 by Western blot at 0.5 - 2 mug/mL.
• Conjugate: Unconjugated
• Immunogen: SHP2 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human SHP2.
• Buffer: SHP2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: SHP2 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of SHP2 in mouse skeletal muscle tissue lysate with SHP2 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL. )

Related Product Information for anti-PTPN11 antibody

SHP2 Antibody: Src homology-2 domain containing protein (SHP2) is a member of the protein tyrosine phosphatase (PTP) family, a protein family that contains signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. SHP2 contains two tandem Src homology-2 (SH2) domains, which function as phosphotyrosine binding domains either directly or through scaffolding intermediates such as the insulin-receptor substrate 1 (IRS-1). These SH2 domains mediate the interaction of SHP2 with its substrates, allowing SHP2 to dephosphorylate proteins that inhibit signaling kinases such as ERK1 and AKT. SHP2 is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Recent experiments have shown SHP2 plays a significant role in hepatoprotection and liver regeneration.

NCBI and Uniprot Product Information

• NCBI GI #: 33356177
• NCBI GeneID: 5781
• NCBI Accession #: NP_002825
• NCBI GenBank Nucleotide #: NM_002834.3
• UniProt Accession #: Q06124; Q96HD7; A8K1D9
• Molecular Weight: 52,828 Da
• NCBI Official Full Name: tyrosine-protein phosphatase non-receptor type 11 isoform 1
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, non-receptor type 11
• NCBI Official Symbol: PTPN11
• NCBI Official Synonym Symbols: CFC; NS1; SHP2; BPTP3; PTP2C; PTP-1D; SH-PTP2; SH-PTP3
• NCBI Protein Information: tyrosine-protein phosphatase non-receptor type 11; PTP-2C; protein-tyrosine phosphatase 1D; protein-tyrosine phosphatase 2C
• UniProt Protein Name: Tyrosine-protein phosphatase non-receptor type 11
• Protein Family: Tyrosine-protein phosphatase non-receptor
• UniProt Gene Name: PTPN11
• UniProt Synonym Gene Names: PTP2C; SHPTP2; PTP-1D; PTP-2C; SHP-2; Shp2
• UniProt Entry Name: PTN11_HUMAN

NCBI Description

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of Noonan syndrome as well as acute myeloid leukemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]

Uniprot Description

SHP-2: a SH2-containing a ubiquitously expressed tyrosine-specific protein phosphatase. It participates in signaling events downstream of receptors for growth factors, cytokines, hormones, antigens and extracellular matrices in the control of cell growth, differentiation, migration, and death. Activation of SHP-2 and its association with Gab1 is critical for sustained Erk activation downstream of several growth factor receptors and cytokines.

Protein type: Motility/polarity/chemotaxis; EC 3.1.3.48; Protein phosphatase, tyrosine (non-receptor)

Chromosomal Location of Human Ortholog: 12q24

Cellular Component: protein complex; mitochondrion; cytoplasm; nucleus; cytosol

Molecular Function: protein domain specific binding; protein binding; phospholipase binding; SH3/SH2 adaptor activity; peptide hormone receptor binding; insulin receptor substrate binding; non-membrane spanning protein tyrosine phosphatase activity; protein tyrosine phosphatase activity; receptor tyrosine kinase binding; phosphoprotein phosphatase activity; D1 dopamine receptor binding; insulin receptor binding

Biological Process: axon guidance; hormone-mediated signaling; nerve growth factor receptor signaling pathway; activation of MAPK activity; heart development; platelet-derived growth factor receptor signaling pathway; positive regulation of mitotic cell cycle; reproductive process in a multicellular organism; glucose homeostasis; negative regulation of insulin secretion; regulation of protein export from nucleus; regulation of cell adhesion mediated by integrin; ephrin receptor signaling pathway; abortive mitotic cell cycle; negative regulation of cell adhesion mediated by integrin; inner ear development; negative regulation of growth hormone secretion; integrin-mediated signaling pathway; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; cytokine and chemokine mediated signaling pathway; positive regulation of hormone secretion; regulation of multicellular organism growth; negative regulation of cortisol secretion; organ growth; genitalia development; triacylglycerol metabolic process; DNA damage checkpoint; T cell costimulation; insulin receptor signaling pathway; innate immune response; hormone metabolic process; platelet formation; brain development; blood coagulation; leukocyte migration

Disease: Metachondromatosis; Noonan Syndrome 1; Juvenile Myelomonocytic Leukemia; Leopard Syndrome 1