Western Blot (WB)
(Western blot analysis of phosphorylated SHIP2 (Y497) in recombinant fusion protein containing 1) a phosphorylated tyrosine at position 497 and 2) an unphosphorylated tyrosine at position 497, using MBS648422 at 0.01ug/ml.)
Rabbit SHIP2 Polyclonal Antibody | anti-INPPL1 antibody
SHIP2, phosphorylated (Tyr497) (Phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase 2, SH2 Domain-containing Inositol-5'-phosphatase 2, SH2 Domain-containing Inositol Phosphatase 2, SHIP-2, Inositol Polyphosphate Phosphatase-like Protein 1, INPPL-1, Pr
Gene Names
INPPL1; SHIP2
Reactivity
Bovine, Canine, Equine, Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
SHIP2; Polyclonal Antibody; phosphorylated (Tyr497) (Phosphatidylinositol-3; 4; 5-trisphosphate 5-phosphatase 2; SH2 Domain-containing Inositol-5'-phosphatase 2; SH2 Domain-containing Inositol Phosphatase 2; SHIP-2; Inositol Polyphosphate Phosphatase-like Protein 1; INPPL-1; Pr; Anti -SHIP2; anti-INPPL1 antibody
Host
Rabbit
Reactivity
Bovine, Canine, Equine, Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human SHIP2 (Y497) phosphorylated. Species Crossreactivity
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Applicable Applications for anti-INPPL1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in Western Blot and Immunohistochemistry.
Dilution: Western Blot: 0.01-0.1ug/ml
Immunohistochemistry (formalin fixed paraffin embedded): 5ug/ml
Dilution: Western Blot: 0.01-0.1ug/ml
Immunohistochemistry (formalin fixed paraffin embedded): 5ug/ml
Immunogen
Synthetic peptide corresponding to human SHIP2 protein containing a phosphorylated tyrosine residue at position 497.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of phosphorylated SHIP2 (Y497) in recombinant fusion protein containing 1) a phosphorylated tyrosine at position 497 and 2) an unphosphorylated tyrosine at position 497, using MBS648422 at 0.01ug/ml.)
Western Blot (WB)
(Western blot analysis of phosphorylated SHIP2 (Y497) in recombinant fusion protein containing 1) a phosphorylated tyrosine at position 497 and 2) an unphosphorylated tyrosine at position 497, using MBS648422 at 0.01ug/ml.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of phosphorylated SHIP2 in formalin-fixed, paraffin-embedded human brain tissue using an isotype control (top) and MBS648422 (bottom) at 5ug/ml.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of phosphorylated SHIP2 in formalin-fixed, paraffin-embedded human brain tissue using an isotype control (top) and MBS648422 (bottom) at 5ug/ml.)
Related Product Information for anti-INPPL1 antibody
SHIP2, also known as 'phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase 2,' is a cytoplasmic protein that translocates to membrane ruffles when activated, due to different stimulus and cell type. It belongs to the inositol-1,4,5-trisphosphate 5-phosphatase family with a SAM (sterile alpha motif) domain and a SH2 domain. SHIP2 specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, thereby negatively regulating the PI3K (phosphoinositide 3-kinase) pathways and also in regulating PI3K-dependent insulin signaling, EGF signaling pathway and a signaling pathway that regulates actin cytoskeleton remodeling. SHIP2 is required for the maintenance and dynamic remodeling of actin structures as well as in endocytosis, having a major impact on ligand-induced EGFR internalization and degradation. It regulates cell adhesion, cell spreading and neuritogenesis and acts as a negative regulator of EPHA2 receptor endocytosis and of the FC-gamma-RIIA receptor (FCGR2A). SHIP2 mediates signaling from the FC-gamma-RIIB receptor (FCGR2B), playing a central role in terminating signal transduction from activating immune/hematopoietic cell receptor systems. Expression is widely seen in most tissues but primarily in skeletal muscle, heart, brain and transformed myeloid cells and in primary macrophages, but not in peripheral blood monocytes.
Product Categories/Family for anti-INPPL1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
SH2 domain-containing inositol-5'-phosphatase 2
NCBI Official Symbol
INPPL1
NCBI Official Synonym Symbols
SHIP2
NCBI Protein Information
phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase 2; SH2 domain-containing inositol-5'-phosphatase 2
Protein Family
Research Articles on INPPL1
Similar Products
Product Notes
The INPPL1 (Catalog #AAA648422) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SHIP2, phosphorylated (Tyr497) (Phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase 2, SH2 Domain-containing Inositol-5'-phosphatase 2, SH2 Domain-containing Inositol Phosphatase 2, SHIP-2, Inositol Polyphosphate Phosphatase-like Protein 1, INPPL-1, Pr reacts with Bovine, Canine, Equine, Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SHIP2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Western Blot and Immunohistochemistry. Dilution: Western Blot: 0.01-0.1ug/ml Immunohistochemistry (formalin fixed paraffin embedded): 5ug/ml. Researchers should empirically determine the suitability of the INPPL1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SHIP2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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