Immunohistochemistry (IHC)
(Cytoplasmic staining of paraffin-embedded rat brain tissue section using MBS627095. Detection using HRP/DAB detection system.)
Rabbit SHH Polyclonal Antibody
SHH (Sonic Hedgehog Protein, HHG-1, Sonic Hedgehog Protein N-product, Sonic Hedgehog Protein C-product)
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
SHH; Polyclonal Antibody; SHH (Sonic Hedgehog Protein; HHG-1; Sonic Hedgehog Protein N-product; Sonic Hedgehog Protein C-product); Anti -SHH (Sonic Hedgehog Protein; anti-SHH antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human SHH. Species Crossreactivity
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with 200ul sterile ddH2O.
Applicable Applications for anti-SHH antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Application Notes
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry.
Dilution: Western Blot: 1-2ug/ml detects SSH in cells and tissues
Immunohistochemistry (FFPE): 1-2ug/ml
Dilution: Western Blot: 1-2ug/ml detects SSH in cells and tissues
Immunohistochemistry (FFPE): 1-2ug/ml
Immunogen
Synthetic peptide corresponding to a sequence near the N-terminal of human SHH. Species Sequence Homology (100%): mouse, rat
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH20. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunohistochemistry (IHC)
(Cytoplasmic staining of paraffin-embedded rat brain tissue section using MBS627095. Detection using HRP/DAB detection system.)
Immunohistochemistry (IHC)
(Cytoplasmic staining of paraffin-embedded rat brain tissue section using MBS627095. Detection using HRP/DAB detection system.)
Western Blot (WB)
(Western Blot analysis of rat brain tissue lysate using MBS627095)
Western Blot (WB)
(Western Blot analysis of rat brain tissue lysate using MBS627095)
Related Product Information for anti-SHH antibody
Sonic Hedgehog Protein (SHH) belongs to hedgehog protein family which includes Indian Hh and Desert Hh. Hh family is involved in the cell fate and patterning during embryonic development, homeostasis and adult tissue renewal. Similar to other member in the family, SHH binds to the patched (PTC) cell surface receptor, releasing the signal transducer Smoothened (Smo) to transmit the Hh signal into the cell and activate transcription of the target gene. Precursor SHH is autocatlytically cleaved into two subunits, N-terminal and C-terminal products. Soluble N-terminal product is involved in the signaling activity, while C-product displays an autoproteolysis activity on the precursor and a cholesterol transferase activity on the N-terminal product. C-terminal product attaches a cholesterol moiety to the N-terminal product, preventing N-terminal product diffusion within the developing embryo. A defect in SHH has been linked in holoprosencephaly type 3 (HPE3), in which developing forebrain fails to separate into right and left hemispheres, and ocular coloboma.
Product Categories/Family for anti-SHH antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The SHH (Catalog #AAA627095) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SHH (Sonic Hedgehog Protein, HHG-1, Sonic Hedgehog Protein N-product, Sonic Hedgehog Protein C-product) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SHH can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC). Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Dilution: Western Blot: 1-2ug/ml detects SSH in cells and tissues Immunohistochemistry (FFPE): 1-2ug/ml. Researchers should empirically determine the suitability of the SHH for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SHH, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
