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Western Blot (WB) (Anti- SHC1 Picoband antibody, MBS178067, Western blottingAll lanes: Anti SHC1 (MBS178067) at 0.5ug/mlLane 1: Rat Thymus Tissue Lysate at 50ugLane 2: Mouse Testis Tissue Lysate at 50ugLane 3: JURKAT Whole Cell Lysate at 40ugLane 4: K562 Whole Cell Lysate at 40ugLane 5: HELA Whole Cell Lysate at 40ugPredicted bind size: 63KDObserved bind size: 63KD)

SHC Polyclonal Antibody | anti-SHC antibody

Anti-SHC Antibody

Gene Names
SHC1; SHC; SHCA
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen Affinity Purified
Synonyms
SHC; Polyclonal Antibody; Anti-SHC Antibody; SHC-transforming protein 1; FLJ26504; MGC98812; p46; p52; p52 SHC; p52SHC; p66; p66 SHC; p66SHC; SH2 domain protein C1; SHC (Src homology 2 domain containing) transforming protein 1; SHC 1; SHC A; Shc; SHC transforming protein 1; SHC transforming protein; SHC-transforming protein 3; SHC-transforming protein A; SHC1; SHC1_HUMAN; SHCA; Src homology 2 domain containing transforming protein C1; Src homology 2 domain-containing-transforming protein C1 antibody; anti-SHC antibody
Ordering
For Research Use Only!
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Purity/Purification
Immunogen Affinity Purified
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
584
Applicable Applications for anti-SHC antibody
Western Blot (WB), Immunohistochemistry (IHC) Formalin/Paraffin
Application Notes
Western Blot Concentration: 0.1-0.5ug/ml
Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml
Immunohistochemistry (IHC) Formalin Concentration: 0.5-1ug/ml
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human SHC (536-564aa VDPEGVVRTKDHRFESVSHLISYHMDNHL), identical to the related mouse and rat sequences.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti- SHC1 Picoband antibody, MBS178067, Western blottingAll lanes: Anti SHC1 (MBS178067) at 0.5ug/mlLane 1: Rat Thymus Tissue Lysate at 50ugLane 2: Mouse Testis Tissue Lysate at 50ugLane 3: JURKAT Whole Cell Lysate at 40ugLane 4: K562 Whole Cell Lysate at 40ugLane 5: HELA Whole Cell Lysate at 40ugPredicted bind size: 63KDObserved bind size: 63KD)

Western Blot (WB) (Anti- SHC1 Picoband antibody, MBS178067, Western blottingAll lanes: Anti SHC1 (MBS178067) at 0.5ug/mlLane 1: Rat Thymus Tissue Lysate at 50ugLane 2: Mouse Testis Tissue Lysate at 50ugLane 3: JURKAT Whole Cell Lysate at 40ugLane 4: K562 Whole Cell Lysate at 40ugLane 5: HELA Whole Cell Lysate at 40ugPredicted bind size: 63KDObserved bind size: 63KD)

Immunohistochemistry (IHC)

(Anti- SHC1 Picoband antibody, MBS178067, IHC(P)IHC(P): Mouse Brain Tissue )

Immunohistochemistry (IHC) (Anti- SHC1 Picoband antibody, MBS178067, IHC(P)IHC(P): Mouse Brain Tissue )

Immunohistochemistry (IHC)

(Anti- SHC1 Picoband antibody, MBS178067, IHC(P)IHC(P): Rat Brain Tissue )

Immunohistochemistry (IHC) (Anti- SHC1 Picoband antibody, MBS178067, IHC(P)IHC(P): Rat Brain Tissue )

Immunohistochemistry (IHC)

(Anti- SHC1 Picoband antibody, MBS178067, IHC(P)IHC(P): Human Lung Cancer Tissue )

Immunohistochemistry (IHC) (Anti- SHC1 Picoband antibody, MBS178067, IHC(P)IHC(P): Human Lung Cancer Tissue )

Immunohistochemistry (IHC)

(Anti- SHC1 Picoband antibody, MBS178067, IHC(F)IHC(F): Human Placenta Tissue )

Immunohistochemistry (IHC) (Anti- SHC1 Picoband antibody, MBS178067, IHC(F)IHC(F): Human Placenta Tissue )
Related Product Information for anti-SHC antibody
Description: Rabbit IgG polyclonal antibody for SHC-transforming protein 1(SHC1) detection. Tested with WB, IHC-P, IHC-F in Human;Mouse;Rat.

Background: SHC, also known as SHC1 (SHC-transforming protein 1) or SHCA, is a protein that in humans is encoded by the SHC1 gene. SCOP classifies the 3D structure as belonging to the SH2 domain family. This gene encodes three main isoforms that differ in activities and subcellular location. While all three are adapter proteins in signal transduction pathways, the longest (p66Shc) may be involved in regulating life span and the effects of reactive oxygen species. The other two isoforms, p52Shc and p46Shc, link activated receptor tyrosine kinases to the Ras pathway by recruitment of the GRB2/SOS complex. p66Shc is not involved in Ras activation. Unlike the other two isoforms, p46Shc is targeted to the mitochondrial matrix. Several transcript variants encoding different isoforms have been found for this gene.
References
1. Harun, R. B., Smith, K. K., Leek, J. P., Markham, A. F., Norris, A., Morrison, J. F. J.Characterization of human SHC p66 cDNA and its processed pseudogene mapping to Xq12-q13.1.Genomics 42: 349-352, 1997. 2. Huebner, K., Kastury, K., Druck, T., Salcini, A. E., Lanfrancone, L., Pelicci, G., Lowenstein, E., Li, W., Park, S.-H., Cannizzaro, L., Pelicci, P. G., Schlessinger, J.Chromosome locations of genes encoding human signal transduction adapter proteins, Nck (NCK), Shc (SHC1), and Grb2 (GRB2).Genomics 22: 281-287, 1994. 3. Pelicci, G., Lanfrancone, L., Grignani, F., McGlade, J., Cavallo, F., Forni, G., Nicoletti, I., Grignani, F., Pawson, T., Pelicci, P. G.A novel transforming protein (SHC) with an SH2 domain is implicated in mitogenic signal transduction.Cell 70: 93-104, 1992.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
51,682 Da
NCBI Official Full Name
SHC-transforming protein 1 isoform 3
NCBI Official Synonym Full Names
SHC (Src homology 2 domain containing) transforming protein 1
NCBI Official Symbol
SHC1
NCBI Official Synonym Symbols
SHC; SHCA
NCBI Protein Information
SHC-transforming protein 1
UniProt Protein Name
SHC-transforming protein 1
Protein Family
UniProt Gene Name
SHC1
UniProt Synonym Gene Names
SHC; SHCA; SH2 domain protein C1
UniProt Entry Name
SHC1_HUMAN

NCBI Description

This gene encodes three main isoforms that differ in activities and subcellular location. While all three are adapter proteins in signal transduction pathways, the longest (p66Shc) may be involved in regulating life span and the effects of reactive oxygen species. The other two isoforms, p52Shc and p46Shc, link activated receptor tyrosine kinases to the Ras pathway by recruitment of the GRB2/SOS complex. p66Shc is not involved in Ras activation. Unlike the other two isoforms, p46Shc is targeted to the mitochondrial matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]

Uniprot Description

SHC1: an adaptor protein containing a SH2 domain and a PID domain within a PH domain-like fold. Couples activated growth factor receptors to signaling pathways. Participates in a signaling cascade initiated by activated KIT and KITLG/SCF. Six human isoforms are produced by alternative promoter usage and alternative splicing. Isoforms p66, p52 and p46 (P29353-1, -2, and -3), produced by alternative initiation, variously regulate growth factor signaling, oncogenesis, intracellular oxidant levels, and apoptosis. Isoforms p46 and p52, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex, thus initiating the cytoplasmic proliferative Ras signaling cascade in various non-neuronal systems. Isoform p66 does not mediate Ras activation, but associates with mitochondria where it controls intracellular redox status, mitochondrial permeability, life span, and stress-induced apoptosis. p66 acts as a downstream target of the tumor suppressor p53 and is required for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. P66 deletion in mice decreases the incidence of aging-associated diseases, such as atherosclerosis, and significantly prolongs life span. Participates in signaling downstream of TIE2, the tyrosine kinase receptor for angiopoietin, and plays a role in the regulation of endothelial cell migration and sprouting angiogenesis. Interacts with tyrosine-phosphorylated CD3T, DDR2, LRP1, IRS4, SHP, FLT4, PDGFRB, TIE2, TrkA, -B and -C. Interacts with the NPXY motif of tyrosine-phosphorylated IGF1R and INSR in vitro via the PID domain. p66Shc is known to be activated by the mutant SOD1 associated with familial forms of amyotrophic lateral sclerosis (ALS), causing a decrease in the activity of Rac1 through a redox-sensitive regulation. In case of oxidative conditions, phosphorylation at S36 of isoform p66Shc, leads to mitochondrial accumulation p66 plays a role in mediating mitophagy and determining neuronal cell fate following acute oxygen glucose deprivation. Isoform p46 is localized to the mitochondria matrix. Targeting of isoform p46Shc to mitochondria is mediated by its first 32 amino acids, which behave as a bona fide mitochondrial targeting sequence. Isoform p52Shc and isoform p66Shc, that contain the same sequence but more internally located, display a different subcellular localization.

Protein type: Apoptosis; Mitochondrial; Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: cytosol; endosome membrane; mitochondrial matrix; nucleus; plasma membrane

Molecular Function: ephrin receptor binding; epidermal growth factor receptor binding; insulin receptor binding; insulin-like growth factor receptor binding; neurotrophin TRKA receptor binding; phospholipid binding; phosphotyrosine binding; protein binding; protein phosphatase 2A binding; Ras guanyl-nucleotide exchange factor activity; transmembrane receptor protein tyrosine kinase adaptor protein activity

Biological Process: actin cytoskeleton reorganization; activation of MAPK activity; aging; angiogenesis; cell-cell adhesion; epidermal growth factor receptor signaling pathway; heart development; insulin receptor signaling pathway; leukocyte migration; MAPKKK cascade; neurite development; organ regeneration; positive regulation of cell proliferation; positive regulation of DNA replication; positive regulation of GTPase activity; positive regulation of smooth muscle cell proliferation; positive regulation of vasoconstriction; Ras protein signal transduction; regulation of epidermal growth factor receptor activity; regulation of growth; response to glucocorticoid stimulus; response to hydrogen peroxide; response to hypoxia; response to nicotine; response to toxin; viral reproduction

Research Articles on SHC

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Product Notes

The SHC shc1 (Catalog #AAA178067) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-SHC Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SHC can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Formalin/Paraffin. Western Blot Concentration: 0.1-0.5ug/ml Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml Immunohistochemistry (IHC) Formalin Concentration: 0.5-1ug/ml. Researchers should empirically determine the suitability of the SHC shc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SHC, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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