Western Blot (WB)
Rabbit Shadow Polyclonal Antibody | anti-sad antibody
Shadow Antibody
Gene Names
sad; 315a1; CG14728; Cyp315a1; CYP315A1; DmelCG14728; Sad
Reactivity
Drosophila melanogaster (Fruit fly)
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Synonyms
Shadow; Polyclonal Antibody; Shadow Antibody; CG14728; Cyp315a1; CYPCCCXVA1; Cytochrome P450 315a1 mitochondrial; Karmoisin ghost; LP13573p; sad; Sad PA; shade antibody; anti-sad antibody
Host
Rabbit
Reactivity
Drosophila melanogaster (Fruit fly)
Clonality
Polyclonal
Form/Format
Affinity Purified
Concentration
0.7 ug/ul in antibody stabilization buffer (varies by lot)
Sequence Length
520
Applicable Applications for anti-sad antibody
Confocal Microscopy (CM), ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
Confocal Microscopy||1:200
Dot Blot: 1:10,000
ELISA: 1:10,000
Immunocytochemistry: 1:200
Immunohistochemistry: 1:200
Immunoprecipitation: 1:200
Western Blot: 1:500
Dot Blot: 1:10,000
ELISA: 1:10,000
Immunocytochemistry: 1:200
Immunohistochemistry: 1:200
Immunoprecipitation: 1:200
Western Blot: 1:500
Immunogen
Synthetic peptide corresponding to amino acids 302-320 of D. melanogaster Shadow. Peptide was conjugated to KLH.
Sequence: CIRVQEDQRRPHDEALYHR
Sequence: CIRVQEDQRRPHDEALYHR
Expression
Complex coexpression pattern of dib (disembodied) and sad (shade) in the early embryo that restricts to the prothoracic gland cells of the developing ring gland during late embryogenesis. In larvae and adult, coexpression is seen in prothoracic gland and follicle cells of the ovary. In adults, coexpression is seen in the follicle cells, sad only is expressed in nurse cells.
Determinant
N-epitope
Molecular Function
Monooxygenase, Oxidoreductase, ecdysteroid 2-hydroxylase activity
Subcellular Location
Mitochondrion membrane
Preparation and Storage
-20 degree C for long term storage
Western Blot (WB)
Western Blot (WB)
Related Product Information for anti-sad antibody
Affinity Purified Shadow Antibody N-epitope
Required for CNS development: midline glial cells. Involved in the metabolism of insect hormones: responsible for ecdysteroid C2-hydroxylase activity. May be involved in the breakdown of synthetic insecticides.
Required for CNS development: midline glial cells. Involved in the metabolism of insect hormones: responsible for ecdysteroid C2-hydroxylase activity. May be involved in the breakdown of synthetic insecticides.
Product Categories/Family for anti-sad antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
59,631 Da
NCBI Official Full Name
shadow
NCBI Official Synonym Full Names
shadow
NCBI Official Symbol
sad
NCBI Official Synonym Symbols
315a1; CG14728; Cyp315a1; CYP315A1; DmelCG14728; Sad
NCBI Protein Information
CG14728 gene product from transcript CG14728-RA
UniProt Protein Name
Cytochrome P450 315a1, mitochondrial
Protein Family
UniProt Gene Name
sad
UniProt Synonym Gene Names
Cyp315a1
UniProt Entry Name
CP315_DROME
Uniprot Description
Required for CNS development: midline glial cells. Involved in the metabolism of insect hormones: responsible for ecdysteroid C2-hydroxylase activity. May be involved in the breakdown of synthetic insecticides.
Research Articles on sad
Similar Products
Product Notes
The sad sad (Catalog #AAA540115) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Shadow Antibody reacts with Drosophila melanogaster (Fruit fly) and may cross-react with other species as described in the data sheet. AAA Biotech's Shadow can be used in a range of immunoassay formats including, but not limited to, Confocal Microscopy (CM), ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB). Confocal Microscopy||1:200 Dot Blot: 1:10,000 ELISA: 1:10,000 Immunocytochemistry: 1:200 Immunohistochemistry: 1:200 Immunoprecipitation: 1:200 Western Blot: 1:500. Researchers should empirically determine the suitability of the sad sad for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Shadow, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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