Rabbit SH3GLB1 Polyclonal Antibody | anti-SH3GLB1 antibody

SH3GLB1 Antibody

Cat. Num. AAA9629768

• Gene Names: SH3GLB1; Bif-1; CGI-61; PPP1R70; dJ612B15.2
• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: SH3GLB1; Polyclonal Antibody; SH3GLB1 Antibody; Bax interacting factor 1; Bax-interacting factor 1; Bif 1; Bif; Bif-1; CGI 61; dJ612B15.2; Endophilin B1; Endophilin-B1; PPP1R70; Protein phosphatase 1 regulatory subunit 70; SH3 containing protein SH3GLB1; SH3 domain GRB2 like endophilin B1; SH3 domain-containing GRB2-like protein B1; Sh3glb1; SH3p13; SHLB1_HUMAN; anti-SH3GLB1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: SH3GLB1 Antibody detects endogenous levels of SH3GLB1.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-SH3GLB1 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human SH3GLB1.
• Conjugation: Unconjugated
• Similarity: An N-terminal amphipathic helix, the BAR domain and a second amphipathic helix inserted into helix 1 of the BAR domain (N-BAR domain) induce membrane curvature and bind curved membranes. The SH3 domain is required and sufficient for the interaction with UVRAG. Belongs to the endophilin family.
• Post Translational Modifications: Phosphorylated at Thr-145 by CDK5; this phosphorylation is required for autophagy induction in starved neurons and facilitates homodimerization.
• Subunit Structure: Homodimer, and heterodimer with SH3GLB2. Binds BAX; induction of apoptosis augments BAX binding. Binds DNM1, HTT, AMPH, BIN1 and ARFGAP1 (By similarity). Interacts with UVRAG; UVRAG bridges the interaction to BECN1 indicative for an association with the PI3K complex II (PI3KC3-C2).
• Tissue Specificity: Highly expressed in heart, skeletal muscle, kidney and placenta. Detected at lower levels in brain, colon, thymus, spleen, liver, small intestine, lung and peripheral blood leukocytes.
• Function: May be required for normal outer mitochondrial membrane dynamics. Required for coatomer-mediated retrograde transport in certain cells (By similarity). May recruit other proteins to membranes with high curvature. May promote membrane fusion. Involved in activation of caspase-dependent apoptosis by promoting BAX/BAK1 activation. Isoform 1 acts proapoptotic in fibroblasts (By similarity). Involved in caspase-independent apoptosis during nutrition starvation and involved in the regulation of autophagy. Activates lipid kinase activity of PIK3C3 during autophagy probably by associating with the PI3K complex II (PI3KC3-C2). Associated with PI3KC3-C2 during autophagy may regulate the trafficking of ATG9A from the Golgi complex to the peripheral cytoplasm for the formation of autophagosomes by inducing Golgi membrane tubulation and fragmentation. Involved in regulation of degradative endocytic trafficking and cytokinesis, probably in the context of PI3KC3-C2. Isoform 2 acts antiapoptotic in neuronal cells; involved in maintenance of mitochondrial morphology and promotes neuronal viability (By similarity).
• Note: Association with the Golgi apparatus depends on the cell type (By similarity). Following starvation colocalizes with ATG5 and LC3 autophagy-related protein(s)on autophagosomal membranes (PubMed:17891140).
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-SH3GLB1 antibody

Cytoplasm; Golgi apparatus membrane; Peripheral membrane protein; Mitochondrion outer membrane; Cytoplasmic vesicle; Autophagosome membrane; Midbody; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 41018158
• NCBI GeneID: 51100
• UniProt Accession #: Q9Y371; Q5H8U5; Q9H3Z0; Q9NR47; Q9NYA9; B4E182
• Molecular Weight: 365
• NCBI Official Full Name: Endophilin-B1
• NCBI Official Synonym Full Names: SH3-domain GRB2-like endophilin B1
• NCBI Official Symbol: SH3GLB1
• NCBI Official Synonym Symbols: Bif-1; CGI-61; PPP1R70; dJ612B15.2
• NCBI Protein Information: endophilin-B1; Bax-interacting factor 1; SH3-containing protein SH3GLB1; SH3 domain-containing GRB2-like protein B1; protein phosphatase 1, regulatory subunit 70
• UniProt Protein Name: Endophilin-B1
• Protein Family: Endophilin
• UniProt Gene Name: SH3GLB1
• UniProt Synonym Gene Names: KIAA0491; Bif-1
• UniProt Entry Name: SHLB1_HUMAN

NCBI Description

This gene encodes a SRC homology 3 domain-containing protein. The encoded protein interacts with the proapoptotic member of the Bcl-2 family, Bcl-2-associated X protein (Bax) and may be involved in regulating apoptotic signaling pathways. This protein may also be involved in maintaining mitochondrial morphology. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011]

Uniprot Description

SH3GLB1: May be required for normal outer mitochondrial membrane dynamics. Required for coatomer-mediated retrograde transport in certain cells. May recruit other proteins to membranes with high curvature. May promote membrane fusion. Binds DNM1, HTT, AMPH, BIN1 and ARFGAP1. Homodimer, and heterodimer with SH3GLB2. Binds BAX. Induction of apoptosis augments BAX binding. Highly expressed in heart, skeletal muscle, kidney and placenta. Detected at lower levels in brain, colon, thymus, spleen, liver, small intestine, lung and peripheral blood leukocytes. Belongs to the endophilin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.3.1.-; Autophagy; Mitochondrial; Transferase; Apoptosis

Chromosomal Location of Human Ortholog: 1p22

Cellular Component: Golgi membrane; mitochondrial outer membrane; cytoplasm

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; lipid binding

Biological Process: positive regulation of protein oligomerization; apoptosis; protein oligomerization