Western Blot (WB)
(Western blot analysis of MBS648073 in Neuro2a cell line lysates (35ug/lane). SH3BGRL3 (arrow) was detected using the purified Pab.)
Rabbit anti-Human SH3BGRL3 Polyclonal Antibody | anti-SH3BGRL3 antibody
SH3BGRL3, ID (SH3BGRL3, SH3 domain-binding glutamic acid-rich-like protein 3, SH3 domain-binding protein 1) (Azide free) (HRP)
Gene Names
SH3BGRL3; TIP-B1; SH3BP-1; HEL-S-297
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
SH3BGRL3; Polyclonal Antibody; ID (SH3BGRL3; SH3 domain-binding glutamic acid-rich-like protein 3; SH3 domain-binding protein 1) (Azide free) (HRP); SH3 domain-binding protein 1; anti-SH3BGRL3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human SH3BGRL3.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-SH3BGRL3 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:50-100
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 24-52 in the C-terminal region of human SH3BGRL3, UniProt Accession #Q9H299. Species sequence homology: bovine
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of MBS648073 in Neuro2a cell line lysates (35ug/lane). SH3BGRL3 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of MBS648073 in Neuro2a cell line lysates (35ug/lane). SH3BGRL3 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain tissue reacted withMBS648073, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain tissue reacted withMBS648073, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.)
Related Product Information for anti-SH3BGRL3 antibody
SH3BGRL3 could act as a modulator of glutaredoxin biological activity.
Product Categories/Family for anti-SH3BGRL3 antibody
References
1. Mazzocco, M., et.al., Biochem. Biophys. Res. Commun. 285 (2), 540-545 (2001). 2. Henn, A.D., et.al., FASEB J. 15 (7), 1315-1317 (2001). 3. Seo, J., et.al., Mol. Cells 10 (6), 733-739 (2000).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
10,438 Da
NCBI Official Full Name
SH3 domain-binding glutamic acid-rich-like protein 3
NCBI Official Synonym Full Names
SH3 domain binding glutamate-rich protein like 3
NCBI Official Symbol
SH3BGRL3
NCBI Official Synonym Symbols
TIP-B1; SH3BP-1; HEL-S-297
NCBI Protein Information
SH3 domain-binding glutamic acid-rich-like protein 3; SH3 domain binding glutamic acid-rich protein like 3; SH3 domain-binding protein 1; SH3BGRL3-like protein; TNF inhibitory protein; epididymis secretory protein Li 297
UniProt Protein Name
SH3 domain-binding glutamic acid-rich-like protein 3
Protein Family
UniProt Gene Name
SH3BGRL3
UniProt Synonym Gene Names
SH3BP-1
UniProt Entry Name
SH3L3_HUMAN
Uniprot Description
SH3BGRL3: Could act as a modulator of glutaredoxin biological activity. Belongs to the SH3BGR family.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 1p36.11
Cellular Component: lamellipodium; cytoplasm; nucleus
Molecular Function: electron carrier activity; protein disulfide oxidoreductase activity; semaphorin receptor binding
Biological Process: cell redox homeostasis; regulation of actin cytoskeleton organization and biogenesis; regulation of blood vessel endothelial cell migration; regulation of actin filament depolymerization
Research Articles on SH3BGRL3
Similar Products
Product Notes
The SH3BGRL3 sh3bgrl3 (Catalog #AAA6347212) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SH3BGRL3, ID (SH3BGRL3, SH3 domain-binding glutamic acid-rich-like protein 3, SH3 domain-binding protein 1) (Azide free) (HRP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SH3BGRL3 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:50-100 ELISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the SH3BGRL3 sh3bgrl3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SH3BGRL3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
