Rabbit SGMS1 Polyclonal Antibody | anti-SGMS1 antibody

SGMS1 Antibody

Cat. Num. AAA9611488

• Reactivity: Human, Mouse, Rat; Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
• Applications: Western Blot
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: SGMS1; Polyclonal Antibody; SGMS1 Antibody; MOB; Phosphatidylcholine:ceramide cholinephosphotransferase 1; Protein Mob; SMS 1; SMS1; TMEM23; Transmembrane protein 23; anti-SGMS1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SGMS1 Antibody detects endogenous levels of SGMS1.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 419

• Applicable Applications for anti-SGMS1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthesized peptide derived from human SGMS1.
• Tissue Specificity: Brain, heart, kidney, liver, muscle and stomach.
• Fragment: Fab Fragment
• Conjugate: Unconjugated
• Subcellular Location: Golgi Apparatus
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from Mouse heart tissue, using SGMS1 Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-SGMS1 antibody

Description: Sphingomyelin synthases synthesize the sphingolipid, sphingomyelin, through transfer of the phosphatidyl head group, phosphatidylcholine, on to the primary hydroxyl of ceramide. The reaction is bidirectional depending on the respective levels of the sphingolipid and ceramide. Golgi apparatus SMS1 directly and specifically recognizes the choline head group on the substrate, requiring two fatty chains on the choline-P donor molecule in order to be recognized efficiently as a substrate. Major form in macrophages. Required for cell growth in certain cell types such as HeLa cells. Suppresses BAX-mediated apoptosis and also prevents cell death in response to stimuli such as hydrogen peroxide, osmotic stress, elevated temperature and exogenously supplied sphingolipids. May protect against cell death by reversing the stress-inducible increase in levels of proapoptotic ceramide.

Similarity: Belongs to the sphingomyelin synthase family.

NCBI and Uniprot Product Information

• NCBI GI #: 44888473
• UniProt Accession #: Q86VZ5
• Molecular Weight: Observed: 50 kDa; Predicted: 49 kDa
• NCBI Official Full Name: Phosphatidylcholine:ceramide cholinephosphotransferase 1
• UniProt Protein Name: Phosphatidylcholine:ceramide cholinephosphotransferase 1
• Protein Family: Phosphatidylcholine:ceramide cholinephosphotransferase
• UniProt Gene Name: SGMS1
• UniProt Synonym Gene Names: MOB; SMS1; TMEM23; Protein Mob
• UniProt Entry Name: SMS1_HUMAN

Uniprot Description

TMEM23: Sphingomyelin synthases synthesize the sphingolipid, sphingomyelin, through transfer of the phosphatidyl head group, phosphatidylcholine, on to the primary hydroxyl of ceramide. The reaction is bidirectional depending on the respective levels of the sphingolipid and ceramide. Golgi apparatus SMS1 directly and specifically recognizes the choline head group on the substrate, requiring two fatty chains on the choline-P donor molecule in order to be recognized efficiently as a substrate. Major form in macrophages. Required for cell growth in certain cell types such as HeLa cells. Suppresses BAX-mediated apoptosis and also prevents cell death in response to stimuli such as hydrogen peroxide, osmotic stress, elevated temperature and exogenously supplied sphingolipids. May protect against cell death by reversing the stress-inducible increase in levels of proapoptotic ceramide. Belongs to the sphingomyelin synthase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; EC 2.7.8.27; Membrane protein, integral; Lipid Metabolism - sphingolipid; Transferase

Chromosomal Location of Human Ortholog: 10q11.2

Cellular Component: Golgi membrane; membrane; endoplasmic reticulum; Golgi trans cisterna; plasma membrane; integral to Golgi membrane; nucleus

Molecular Function: sphingomyelin synthase activity; kinase activity; ceramide cholinephosphotransferase activity

Biological Process: sphingolipid metabolic process; sphingolipid biosynthetic process; apoptosis; cell growth; sphingomyelin biosynthetic process; inflammatory response; phosphorylation