anti-Mouse SGLT-3 Polyclonal Antibody | anti-SGLT-3 antibody

Anti - SGLT-3 (Sodium Glucose Transporter 3)

Cat. Num. AAA612551

• Reactivity: Mouse
• Applications: ELISA, Western Blot
• Purity: Serum
• Synonyms: SGLT-3; Polyclonal Antibody; Anti - SGLT-3 (Sodium Glucose Transporter 3); SGLT-3 (Sodium Glucose Transporter 3); anti-SGLT-3 antibody

• Host: Host: Rabbit; Source: Mouse
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: The mouse peptide sequence is poorly conserved (~37%) in human and porcine SGLT3. No significant sequence homology exists with other SGLTs. For human SGLT3, we recommend the use antibody and SG-34 or SG35 for porcine SGLT-3 that is made to the human and porcine SGLT-3 peptide, respectively. Control peptide, because of its low MW (<3kD), is not suitable for Western. It should be used for ELISA or antibody blocking experiments (use 5-10 ug control peptide per 1 ug of aff pure IgG or 1 ul antiserum) to confirm antibody specificity.
• Purity/Purification: Serum
• Form/Format: Serum; Supplied as a liquid, neat serum, 40% glycerol.

• Applicable Applications for anti-SGLT-3 antibody: ELISA, Western Blot
• Application Notes: Suitable for use in ELISA, Western Blotting. Other applications not tested.; Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure antibody using ECL.; ELISA: 1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity pure. Control peptide can be used to coat ELISA plates at 1ug/ml.; Optimal dilutions to be determined by the researcher.
• Immunogen: A 16 AA peptide sequence from the N-terminal, cytoplasmic domain 1 of Mouse SGLT-3 (1) was synthesized, coupled to KLH
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-SGLT-3 antibody

The kidneys play a major role in the regulation of glucose levels. Kidneys filter approx. 180 g of glucose per day from the blood, and this is mostly reabsorbed back into the blood in the proximal tubules. Typically, glucose is first absorbed within epithelium by a specific transporter protein, Sodium glucoseco transporters (SGLT), in the brush-border membrane and then it is transported out of the cell across the basolateral membranes by a facilitated sugar transporter (GLUTs). At least 3 members of SGLTs (SGLT1-3) have been cloned and characterized from various species. Individual member of this family have identical predicted secondary structures with up to 14 transmembrane domains. SGLT1-3 genes code for protein of approx 659-672 residues (calculated size of ~75kD). Both N and C-termini are predicted to be extracellualr. There is approx 60-70% homology between SGLT1-3. SGLTs transport a-methyl-D-glucoside (a-MDG), a non-metabolized model substrate, in Na-dependent manner. SGLT1 does not discriminate a-MDG, glucose, and galactose. SGLT2/3 do not transport D-galactose efficiently.

Product Categories/Family for anti-SGLT-3 antibody

Abs to Glucose Transporters

References

• 1. Tatabai Nm et al (2001) Toxicol. Appl. Pharamcol. 177, 163-177; Kong CT et al (1993) JBC 268, 1509-1512; Mackenzie B et al (1994) JBC 269, 22488-22491; Dunham I et al (1999) Nature 402, 489-495; Wright E (2001) Am. J. Physiol. Renal Physiol. 280, F10-F18 (review).