anti-Rat SGLT-1 Polyclonal Antibody | anti-SGLT-1 antibody
Anti - SGLT-1 (Sodium Glucose Transporter 1)
Reactivity
Rat
Applications
ELISA, Western Blot
Purity
Serum
Synonyms
SGLT-1; Polyclonal Antibody; Anti - SGLT-1 (Sodium Glucose Transporter 1); SGLT-1 (Sodium Glucose Transporter 1); anti-SGLT-1 antibody
Host
Host: Rabbit
Source: Rat
Source: Rat
Reactivity
Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
The rat peptide sequence is 93% conserved in mouse and 50% in human SGLT1. No significant sequence homology exists with other SGLTs. For human, we recommend the use antibody that is made to the human SGLT-1 peptide. Control peptide, because of its low MW (<3kD), is not suitable for Western. It should be used for ELISA or antibody blocking experiments (use 5-10 ug control peptide per 1 ug of aff pure IgG or 1 ul antiserum) to confirm antibody specificity.
Purity/Purification
Serum
Form/Format
Serum
Supplied as a liquid, neat serum, 40% glycerol.
Supplied as a liquid, neat serum, 40% glycerol.
Applicable Applications for anti-SGLT-1 antibody
ELISA, Western Blot
Application Notes
Suitable for use in ELISA, Western Blotting. Other applications not tested.
Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL. A band of 95-110kD has been detected in brush border membrane (2).
ELISA:1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity purified. Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL. A band of 95-110kD has been detected in brush border membrane (2).
ELISA:1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity purified. Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
Immunogen
A 16 AA peptide sequence of rat SGLT-1 from the N-terminal, cytoplasmic domain 1 (1) was synthesized, coupled to KLH
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Product Categories/Family for anti-SGLT-1 antibody
References
• 1. ; Lee WS et al (1994) JBC 269, 12032, Gene accession # AF163846;
• 2. Hediger, MA et al (1987) Nature 330, 1379-1381;
• 3. Sileverman, M et al (1993) BBA 1153, 43-52; Pajor, AM et al (1992) Am. J. Physiol. 32, R489-R495; Hirayama, EA et al (1992) BBA 1103, 37-44; Hirayama et al (1991) Am. J. Physiol. 261, C296; Wright EM (2001) Am. J. Renal Physiol. 280, F10 (review).
• 2. Hediger, MA et al (1987) Nature 330, 1379-1381;
• 3. Sileverman, M et al (1993) BBA 1153, 43-52; Pajor, AM et al (1992) Am. J. Physiol. 32, R489-R495; Hirayama, EA et al (1992) BBA 1103, 37-44; Hirayama et al (1991) Am. J. Physiol. 261, C296; Wright EM (2001) Am. J. Renal Physiol. 280, F10 (review).
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Product Notes
The SGLT-1 (Catalog #AAA616218) is an Antibody produced from Host: Rabbit Source: Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti - SGLT-1 (Sodium Glucose Transporter 1) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SGLT-1 can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot. Suitable for use in ELISA, Western Blotting. Other applications not tested. Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL. A band of 95-110kD has been detected in brush border membrane (2). ELISA:1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity purified. Control peptide can be used to coat ELISA plates at 1ug/ml. Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the SGLT-1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SGLT-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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