Rabbit anti-Human, Mouse SGIP1 Polyclonal Antibody | anti-SGIP1 antibody

SGIP1, NT (SGIP1, SH3-containing GRB2-like protein 3-interacting protein 1, Endophilin-3-interacting protein) (AP)

Cat. Num. AAA6346867

• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SGIP1; Polyclonal Antibody; NT (SGIP1; SH3-containing GRB2-like protein 3-interacting protein 1; Endophilin-3-interacting protein) (AP); Endophilin-3-interacting protein; anti-SGIP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-SGIP1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: SGIP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human SGIP1.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(SGIP1 Antibody (N-term) western blot analysis in CHO,MDA-MB231, mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the SGIP1 antibody detected the SGIP1 protein (arrow).)

Related Product Information for anti-SGIP1 antibody

SGIP1 functions as an endocytic protein that affects signaling by receptors in neuronal systems involved in energy homeostasis via its interaction with endophilins (see SH3GL3; MIM 603362) (Trevaskis et al., 2005 [PubMed 15919751] and Uezu et al., 2007 [PubMed 17626015]).

Product Categories/Family for anti-SGIP1 antibody

Antibodies; Proteins

References

Luke, M.M., et al. Stroke 40(2):363-368(2009). Shiffman, D., et al. Arterioscler. Thromb. Vasc. Biol. 28(1):173-179(2008). Uezu, A., et al. J. Biol. Chem. 282(36):26481-26489(2007). Trevaskis, J., et al. Endocrinology 146(9):3757-3764(2005). Simpson, J.C., et al. EMBO Rep. 1(3):287-292(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 815890969
• NCBI GeneID: 84251
• NCBI Accession #: NP_001295132.1
• NCBI GenBank Nucleotide #: NM_001308203.1
• UniProt Accession #: Q9BQI5; Q4LE32; Q5VYE2; Q5VYE3; Q5VYE4; Q68D76; Q6MZY6; Q8IWC2; A6NL81; A6NLD1
• Molecular Weight: 68,338 Da
• NCBI Official Full Name: SH3-containing GRB2-like protein 3-interacting protein 1 isoform 2
• NCBI Official Synonym Full Names: SH3-domain GRB2-like (endophilin) interacting protein 1
• NCBI Official Symbol: SGIP1
• NCBI Protein Information: SH3-containing GRB2-like protein 3-interacting protein 1
• UniProt Protein Name: SH3-containing GRB2-like protein 3-interacting protein 1
• Protein Family: SH3-containing GRB2-like protein 3-interacting protein
• UniProt Gene Name: SGIP1
• UniProt Entry Name: SGIP1_HUMAN

NCBI Description

SGIP1 functions as an endocytic protein that affects signaling by receptors in neuronal systems involved in energy homeostasis via its interaction with endophilins (see SH3GL3; MIM 603362) (Trevaskis et al., 2005 [PubMed 15919751] and Uezu et al., 2007 [PubMed 17626015]).[supplied by OMIM, Mar 2008]

Uniprot Description

SGIP1: May function in clathrin-mediated endocytosis. Has both a membrane binding/tubulating activity and the ability to recruit proteins essential to the formation of functional clathrin-coated pits. Has a preference for membranes enriched in phosphatidylserine and phosphoinositides and is required for the endocytosis of the transferrin receptor. May also bind tubulin. May play a role in the regulation of energy homeostasis. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 1p31.3

Cellular Component: clathrin-coated vesicle; cytoplasm; plasma membrane; AP-2 adaptor complex; coated pit

Molecular Function: protein binding; microtubule binding; phospholipid binding; SH3 domain binding

Biological Process: response to dietary excess; endocytosis; positive regulation of receptor-mediated endocytosis