Rabbit anti-Human SFRS8 Polyclonal Antibody | anti-SFRS8 antibody

SFRS8, NT (SFSWAP, SFRS8, SWAP, Splicing factor, suppressor of white-apricot homolog, Splicing factor, arginine/serine-rich 8, Suppressor of white apricot protein homolog) (Azide free) (HRP)

Cat. Num. AAA6346717

• Gene Names: SFSWAP; SWAP; SFRS8
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SFRS8; Polyclonal Antibody; NT (SFSWAP; SWAP; Splicing factor; suppressor of white-apricot homolog; arginine/serine-rich 8; Suppressor of white apricot protein homolog) (Azide free) (HRP); SFSWAP; Suppressor of white apricot protein homolog; anti-SFRS8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Sequence Length: 951

• Applicable Applications for anti-SFRS8 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: SFRS8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human SFRS8.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SFRS8 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the SFRS8 antibody detected the SFRS8 protein (arrow).)

Related Product Information for anti-SFRS8 antibody

This gene encodes a human homolog of Drosophila splicing regulatory protein. This gene autoregulates its expression by control of splicing of its first two introns. In addition, it also regulates the splicing of fibronectin and CD45 genes.

Product Categories/Family for anti-SFRS8 antibody

Antibodies; Splicing Factors

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Matsuoka, S., et al. Science 316(5828):1160-1166(2007). Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007): Olsen, J.V., et al. Cell 127(3):635-648(2006). Olsen, J.V., et al. Cell 127(3):635-648(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 23111062
• NCBI GeneID: 6433
• NCBI Accession #: NP_004583.2
• NCBI GenBank Nucleotide #: NP_004583.2
• UniProt Accession #: Q12872
• NCBI Official Full Name: splicing factor, suppressor of white-apricot homolog isoform 2
• NCBI Official Synonym Full Names: splicing factor SWAP
• NCBI Official Symbol: SFSWAP
• NCBI Official Synonym Symbols: SWAP; SFRS8
• NCBI Protein Information: splicing factor, suppressor of white-apricot homolog
• UniProt Protein Name: Splicing factor, suppressor of white-apricot homolog
• UniProt Gene Name: SFSWAP
• UniProt Synonym Gene Names: SFRS8; SWAP
• UniProt Entry Name: SFSWA_HUMAN

NCBI Description

This gene encodes a human homolog of Drosophila splicing regulatory protein. This gene autoregulates its expression by control of splicing of its first two introns. In addition, it also regulates the splicing of fibronectin and CD45 genes. Two transcript variants encoding different isoforms have been identified. [provided by RefSeq, May 2012]

Uniprot Description

SFRS8: Plays a role as an alternative splicing regulator. Regulate its own expression at the level of RNA processing. Also regulates the splicing of fibronectin and CD45 genes. May act, at least in part, by interaction with other R/S-containing splicing factors. Represses the splicing of MAPT/Tau exon 10.

Protein type: RNA splicing; RNA-binding

Chromosomal Location of Human Ortholog: 12q24.33

Cellular Component: nucleus

Molecular Function: RNA binding

Biological Process: mRNA splice site selection; negative regulation of nuclear mRNA splicing, via spliceosome; transcription, DNA-dependent; regulation of transcription, DNA-dependent; mRNA processing