Rabbit anti-Human SFRS2 Polyclonal Antibody | anti-SFRS2 antibody

SFRS2, NT (Splicing Factor, Arginine/Serine-Rich 2, Splicing Factor SC35, SC-35, Splicing Component, 35kD, Protein PR264) (PE)

Cat. Num. AAA6500534

• Gene Names: SRSF2; SC35; PR264; SC-35; SFRS2; SFRS2A; SRp30b
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: SFRS2; Polyclonal Antibody; NT (Splicing Factor; Arginine/Serine-Rich 2; Splicing Factor SC35; SC-35; Splicing Component; 35kD; Protein PR264) (PE); anti-SFRS2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SFRS2.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-SFRS2 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human SFRS2 (KLH).
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-SFRS2 Antibody (N-term) in K562 cell line lysates (35ug/lane).SFRS2(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SFRS2 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-SFRS2 antibody

Antibodies; Splicing Factors

References

1. Merdzhanova, G., Cell Death Differ. 15(12), 1815-1823 (2008). 2. Solis, A.S., J. Biol. Chem. 283(35), 23619-23626 (2008). 3. Donev, R., Mol. Psychiatry 12(7), 681-690 (2007). 4. Sureau, A., Proc. Natl. Acad. Sci. USA 89(24), 11683-11687 (1992).

NCBI and Uniprot Product Information

• NCBI GI #: 47271443
• NCBI GeneID: 6427
• NCBI Accession #: NP_003007
• NCBI GenBank Nucleotide #: NM_003016
• UniProt Accession #: Q01130
• Molecular Weight: 25kDa
• NCBI Official Full Name: serine/arginine-rich splicing factor 2
• NCBI Official Synonym Full Names: serine and arginine rich splicing factor 2
• NCBI Official Symbol: SRSF2
• NCBI Official Synonym Symbols: SC35; PR264; SC-35; SFRS2; SFRS2A; SRp30b
• NCBI Protein Information: serine/arginine-rich splicing factor 2
• UniProt Protein Name: Serine/arginine-rich splicing factor 2
• UniProt Gene Name: SRSF2
• UniProt Synonym Gene Names: SFRS2; SC-35
• UniProt Entry Name: SRSF2_HUMAN

NCBI Description

The protein encoded by this gene is a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors, which constitute part of the spliceosome. Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation. Two transcript variants encoding the same protein and one non-coding transcript variant have been found for this gene. In addition, a pseudogene of this gene has been found on chromosome 11. [provided by RefSeq, Sep 2010]

Uniprot Description

SFRS2: Necessary for the splicing of pre-mRNA. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre- mRNA. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3'. Can bind to beta-globin mRNA and commit it to the splicing pathway. The phosphorylated form (by SRPK2) is required for cellular apoptosis in response to cisplatin treatment. Belongs to the splicing factor SR family.

Protein type: RNA processing; RNA splicing; Spliceosome

Chromosomal Location of Human Ortholog: 17q25.1

Cellular Component: nucleoplasm; spliceosome; PML body; cytoplasm; nuclear speck; nucleus

Molecular Function: protein binding; nucleotide binding; transcription corepressor activity

Biological Process: nuclear mRNA splicing, via spliceosome; transcription from RNA polymerase II promoter; mRNA export from nucleus; RNA splicing; gene expression; mRNA 3'-end processing; mRNA processing; termination of RNA polymerase II transcription