Rabbit SF3B14 Polyclonal Antibody | anti-SF3B14 antibody

SF3B14 Polyclonal Antibody

Cat. Num. AAA3007608

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: SF3B14; Polyclonal Antibody; SF3B14 Polyclonal Antibody; Splicing factor 3B subunit 6; Pre-mRNA branch site protein p14; SF3b 14 kDa subunit; SF3B14a; Spliceosome-associated protein; 14-kDa; Splicing factor 3b; subunit 6; 14kDa; SF3B6; SAP14; SF3B14A; CGI-110; HSPC175; HT006; anti-SF3B14 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SF3B14 polyclonal antibody detects endogenous levels of SF3B14 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 125

• Applicable Applications for anti-SF3B14 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 70-120 of Human SF3B14.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of SF3B14 polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysate (40ug) Lane2:HepG2 whole cell lysate (40ug) Lane3:AML-12 whole cell lysate (40ug) Lane4:PC12 whole cell lysate (40ug))

Related Product Information for anti-SF3B14 antibody

Splicing factor 3B subunit 6, necessary for the splicing of pre-mRNA. Directly contacts the pre-mRNA branch site adenosine for the first catalytic step of splicing. Enters the spliceosome and associates with the pre-mRNA branch site as part of the 17S U2 or, in the case of the minor spliceosome, as part of the 18S U11/U12 snRNP complex, and thus may facilitate the interaction of these snRNP with the branch sites of U2 and U12 respectively.

NCBI and Uniprot Product Information

• NCBI GI #: 7706326
• NCBI Accession #: NP_057131.1
• NCBI GenBank Nucleotide #: NP_057131.1
• UniProt Accession #: Q9Y3B4
• Molecular Weight: ~ 15kDa
• NCBI Official Full Name: splicing factor 3B subunit 6
• UniProt Protein Name: Pre-mRNA branch site protein p14
• UniProt Gene Name: SF3B14
• UniProt Entry Name: PM14_HUMAN

Uniprot Description

SF3B6: subunit of the splicing factor SF3B. Necessary for the splicing of pre-mRNA. Directly contacts the pre-mRNA branch site adenosine for the first catalytic step of splicing. Enters the spliceosome and associates with the pre-mRNA branch site as part of the 17S U2 or, in the case of the minor spliceosome, as part of the 18S U11/U12 snRNP complex, and thus may facilitate the interaction of these snRNP with the branch sites of U2 and U12 respectively.

Protein type: RNA-binding; RNA splicing; Spliceosome

Chromosomal Location of Human Ortholog: 2p23

Cellular Component: nucleoplasm; U12-dependent spliceosome

Molecular Function: nucleotide binding

Biological Process: nuclear mRNA splicing, via spliceosome; RNA splicing; gene expression