Rabbit anti-Human, Mouse SF3A1 Polyclonal Antibody | anti-SF3A1 antibody
SF3A1 Antibody
Gene Names
SF3A1; PRP21; PRPF21; SAP114; SF3A120
Reactivity
Human, Mouse
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
SF3A1; Polyclonal Antibody; SF3A1 Antibody; Pre mRNA processing 21; Pre mRNA splicing factor SF3a; PRP21; PRPF21; SAP 114; SAP114; sf3a1; SF3A1_HUMAN; SF3a120; Spliceosome associated protein 114; Spliceosome-associated protein 114; Splicing factor 3 subunit 1; Splicing factor 3a subunit 1 120kDa; Splicing factor 3A subunit 1; anti-SF3A1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
SF3A1 Antibody detects endogenous levels of SF3A1.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-SF3A1 antibody
ELISA (EIA)(peptide)
Application Notes
ELISA(peptide): 1:20000-1:40000
Immunogen
A synthesized peptide derived from human SF3A1.
Conjugation
Unconjugated
Similarity
SURP motif 2 mediates direct binding to SF3A3.
Subunit Structure
Component of splicing factor SF3A which is composed of three subunits; SF3A3/SAP61, SF3A2/SAP62 and SF3A1/SAP114. SF3A1 functions as scaffold that interacts directly with both SF3A2 and SF3A3. SF3A associates with the splicing factor SF3B and a 12S RNA unit to form the mature 17S U2 small nuclear ribonucleoprotein complex (17S U2 snRNP). Identified in the spliceosome 'E' complex, a precursor of the spliceosome 'A' complex. Identified in the spliceosome 'A' and 'B' complexes. Identified in the spliceosome 'C' complex.
Tissue Specificity
Ubiquitously expressed.
Function
Involved in pre-mRNA splicing as a component of the splicing factor SF3A complex that contributes to the assembly of the 17S U2 snRNP, and the subsequent assembly of the pre-spliceosome 'E' complex and the pre-catalytic spliceosome 'A' complex. Involved in pre-mRNA splicing as a component of pre-catalytic spliceosome 'B' complexes.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Product Categories/Family for anti-SF3A1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
793
NCBI Official Full Name
Splicing factor 3A subunit 1
NCBI Official Synonym Full Names
splicing factor 3a, subunit 1, 120kDa
NCBI Official Symbol
SF3A1
NCBI Official Synonym Symbols
PRP21; PRPF21; SAP114; SF3A120
NCBI Protein Information
splicing factor 3A subunit 1; SAP 114; pre-mRNA processing 21; splicing factor 3 subunit 1; spliceosome-associated protein 114; splicing factor 3a, subunit 1, 120kD; pre-mRNA splicing factor SF3a (120 kDa subunit)
UniProt Protein Name
Splicing factor 3A subunit 1
Protein Family
UniProt Gene Name
SF3A1
UniProt Synonym Gene Names
SAP114; SAP 114
UniProt Entry Name
SF3A1_HUMAN
NCBI Description
This gene encodes a subunit of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer is a component of the mature U2 small nuclear ribonucleoprotein particle (snRNP). U2 small nuclear ribonucleoproteins play a critical role in spliceosome assembly and pre-mRNA splicing. [provided by RefSeq, Aug 2014]
Uniprot Description
SF3A1: subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex.
Protein type: RNA-binding; Nuclear receptor co-regulator; Spliceosome; RNA splicing
Chromosomal Location of Human Ortholog: 22q12.2
Cellular Component: nucleoplasm; spliceosome
Molecular Function: protein binding; RNA binding
Biological Process: nuclear mRNA 3'-splice site recognition; nuclear mRNA splicing, via spliceosome; RNA splicing; gene expression; mRNA processing
Research Articles on SF3A1
Similar Products
Product Notes
The SF3A1 sf3a1 (Catalog #AAA9630992) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SF3A1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SF3A1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA)(peptide). ELISA(peptide): 1:20000-1:40000. Researchers should empirically determine the suitability of the SF3A1 sf3a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SF3A1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
