Rabbit anti-Human SET, Phosphatase 2A Inhibitor I2PP2A Polyclonal Antibody | anti-SET antibody

SET, Phosphatase 2A Inhibitor I2PP2A

Cat. Num. AAA395860

• Gene Names: SET; 2PP2A; IGAAD; TAF-I; I2PP2A; IPP2A2; PHAPII; TAF-IBETA
• Reactivity: Human
• Applications: Western Blot
• Purity: Antigen Immunoaffiinity Purification
• Synonyms: SET; Phosphatase 2A Inhibitor I2PP2A; Polyclonal Antibody; SET translocation (myeloid leukemia-associated); Protein SET; Phosphatase 2A inhibitor; I2PP2A; I-2PP2A; Template-activating factor I; TAF-I; HLA-DR-associated protein II; PHAPII; Inhibitor of granzyme A-activated DNase; IGAAD2PP2A; IGAAD; TAF-IBETA; anti-SET antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Immunoaffiinity Purification
• Form/Format: Provided as solution in phosphate buffered saline with 0.08% sodium azide
• Sequence Length: 290

• Applicable Applications for anti-SET antibody: Western Blot (WB)
• Immunogen: Synthetic peptide derived from the human SET protein
• Positive Control: Widely expressed. Low levels in quiescent cells during serum starvation, contact inhibition or differentiation. Highly expressed in Wilms' tumor.
• Preparation and Storage: Product should be stored at -70 degree C. Aliquot to avoid freeze/thaw cycles

Related Product Information for anti-SET antibody

Human SET was originally identified as part of the SET-CAN fusion gene produced by a somatic translocation event in a patient with acute undifferentiated leukemia. In developing kidney, SET is highly expressed in the zone of nephron morphogenesis. SET has been shown to be a potent and specific inhibitor of protein phosphatase 2A, a family of major serine/threonine phosphatases involved in regulating cell proliferation and differentiation. SET is also involved in the regulation of renal cell proliferation and tumorigenesis. SET mRNA expression is markedly reduced in cells rendered quiescent by serum starvation, contact inhibition, or differentiation. SET protein expression is also much greater in developing rat and human kidney than in fully differentiated, mature kidney. High levels of SET mRNA and SET protein expression arefound in Wilms' tumor, but not in renal cell carcinoma, adult polycystic kidney disease or in transitional cell carcinoma.

Product Categories/Family for anti-SET antibody

Antigen Immunoaffinity Purified Polyclonal; Protein Phosphatases

NCBI and Uniprot Product Information

• NCBI GI #: 170763500
• NCBI GeneID: 6418
• NCBI Accession #: NP_001116293.1
• NCBI GenBank Nucleotide #: NM_001122821.1
• UniProt Accession #: Q01105; Q15541; Q5VXV1; Q5VXV2; Q6FHZ5; A5A5H4; A6NGV1; B4DUE2
• Molecular Weight: 33489
• NCBI Official Full Name: protein SET isoform 1
• NCBI Official Synonym Full Names: SET nuclear oncogene
• NCBI Official Symbol: SET
• NCBI Official Synonym Symbols: 2PP2A; IGAAD; TAF-I; I2PP2A; IPP2A2; PHAPII; TAF-IBETA
• NCBI Protein Information: protein SET; HLA-DR-associated protein II; phosphatase 2A inhibitor I2PP2A; inhibitor-2 of protein phosphatase-2A; protein phosphatase type 2A inhibitor; inhibitor of granzyme A-activated DNase; SET translocation (myeloid leukemia-associated); Template-Activating Factor-I, chromatin remodelling factor
• UniProt Protein Name: Protein SET
• Protein Family: Protein
• UniProt Gene Name: SET
• UniProt Synonym Gene Names: IGAAD; I-2PP2A; TAF-I
• UniProt Entry Name: SET_HUMAN

NCBI Description

The protein encoded by this gene inhibits acetylation of nucleosomes, especially histone H4, by histone acetylases (HAT). This inhibition is most likely accomplished by masking histone lysines from being acetylated, and the consequence is to silence HAT-dependent transcription. The encoded protein is part of a complex localized to the endoplasmic reticulum but is found in the nucleus and inhibits apoptosis following attack by cytotoxic T lymphocytes. This protein can also enhance DNA replication of the adenovirus genome. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

Function: Multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone chaperoning. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher.

Subunit structure: Interacts with CHTOP

By similarity. Headphone-shaped homodimer. Isoform 1 and isoform 2 interact directly with each other and with ANP32A within the tripartite INHAT (inhibitor of acetyltransferases) complex. Isoform 1 and isoform 2 interact also with histones. Isoform 2 is a component of the SET complex, which also contains ANP32A, APEX1, HMGB2 and NME1, but not NME2. Within this complex, directly interacts with NME1 and with HMGB2. Interacts with SETBP1. Interacts with SGOL1. Interacts with APBB1. Ref.15 Ref.16 Ref.21 Ref.25 Ref.30

Subcellular location: Cytoplasm › cytosol. Endoplasmic reticulum. Nucleus › nucleoplasm. Note: In the cytoplasm, found both in the cytosol and associated with the endoplasmic reticulum. Following CTL attack, moves rapidly to the nucleus, where it is found in the nucleoplasm, avoiding the nucleolus. Similar translocation to the nucleus is also observed for lymphocyte-activated killer cells after the addition of calcium. The SET complex is associated with the endoplasmic reticulum. Ref.17 Ref.19

Tissue specificity: Widely expressed. Low levels in quiescent cells during serum starvation, contact inhibition or differentiation. Highly expressed in Wilms' tumor.

Domain: A long alpha helix in the N-terminus mediates dimerization, while the earmuff domain is responsible for core histone and dsDNA binding. The C-terminal acidic domain mediates the inhibition of histone acetyltransferases and is required for the DNA replication stimulatory activity. Ref.30

Post-translational modification: Isoform 2 is phosphorylated on Ser-15 and Thr-23.Isoform 2 is acetylated on Lys-11.Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group

By similarity.N-terminus of isoform 1 is methylated by METTL11A/NTM1. Mainly trimethylated

By similarity.

Involvement in disease: A chromosomal aberration involving SET is found in some cases of acute undifferentiated leukemia (AUL). Translocation t(6;9)(q21;q34.1) with NUP214/CAN.

Sequence similarities: Belongs to the nucleosome assembly protein (NAP) family.