Rabbit anti-Mouse Serum Amyloid P Polyclonal Antibody | anti-SAP antibody

Serum Amyloid P (9.5S alpha 1 Glycoprotein, 9.5S alpha-1-glycoprotein, Amyloid P Component Serum, APCS, Pentaxin Related, Pentraxin 2, PTX 2, PTX2, SAMP_HUMAN, SAP, Serum Amyloid P Component, Serum Amyloid P-component(1-203)) (FITC)

Cat. Num. AAA6460752

• Reactivity: Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: Serum Amyloid P; Polyclonal Antibody; Serum Amyloid P (9.5S alpha 1 Glycoprotein; 9.5S alpha-1-glycoprotein; Amyloid P Component Serum; APCS; Pentaxin Related; Pentraxin 2; PTX 2; PTX2; SAMP_HUMAN; SAP; Serum Amyloid P Component; Serum Amyloid P-component(1-203)) (FITC); anti-SAP antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse Serum Amyloid P.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-SAP antibody: Immunohistochemistry (IHC) Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: IHC-P: 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.; ELISA: 0.1-0.5ug/ml; WB: 0.1-0.5ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to mouse Serum Amyloid P expressed in E. coli. (Position: Q21-D224). Species sequence homology: human (70%) and rat (80%)
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months after receipt at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunohistochemistry (IHC)

(IHC(P): Mouse Kidney Tissue)

Western Blot (WB)

(Anti-Serum Amyloid P Picoband antibody, All lanes: Anti-Serum Amyloid P at 0.5ug/ml WB: Recombinant Mouse Serum Amyloid P Protein 0.5ng Predicted band size: 39KD Observed band size: 39KD)

Product Categories/Family for anti-SAP antibody

Antibodies; Amyloid

References

1. Cathcart ES, Shirahama T, Cohen AS (1967). "Isolation and identification of a plasma component of amyloid". Biochim. Biophy. Acta 147: 392-393. 2. "Entrez Gene: APCS amyloid P component, serum". 3. Emsley J, White HE, O'Hara BP, Oliva G, Srinivasan N, Tickle IJ, Blundell TL, Pepys MB, Wood SP (1994). "Structure of pentameric human serum amyloid P component". Nature 367(6461): 338-45.

NCBI and Uniprot Product Information

• NCBI GI #: 134198
• UniProt Accession #: P12246
• NCBI Official Full Name: Serum amyloid P-component
• UniProt Protein Name: Serum amyloid P-component
• Protein Family: S-layer protein
• UniProt Gene Name: Apcs
• UniProt Synonym Gene Names: Ptx2; Sap; SAP
• UniProt Entry Name: SAMP_MOUSE

Uniprot Description

APCS: Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. May also function as a calcium-dependent lectin. SAP is a precursor of amyloid component P which is found in basement membrane and associated with amyloid deposits. Belongs to the pentaxin family.

Protein type: Secreted; Secreted, signal peptide; Chaperone

Cellular Component: extracellular matrix; extracellular space; protein complex; extracellular region; nucleus

Molecular Function: metal ion binding; complement component C1q binding; virion binding; calcium ion binding; carbohydrate binding

Biological Process: negative regulation of virion penetration into host cell; innate immune response; protein complex assembly; negative regulation of monocyte differentiation; negative regulation of viral reproduction