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Western Blot (WB) (Western Blot analysis of Septin 2 in HepG2 cells using Septin 2 Rabbit pAb at dilution 1/400)

Rabbit Septin 2 Polyclonal Antibody | anti-SEPTIN2 antibody

Septin 2 Rabbit pAb

Gene Names
SEPT2; DIFF6; NEDD5; Pnutl3; hNedd5
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purified
Synonyms
Septin 2; Polyclonal Antibody; Septin 2 Rabbit pAb; septin 2; DIFF6; NEDD5; SEPT2; NEDD-5; Pnutl3; hNedd5; anti-SEPTIN2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purified
Form/Format
pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Concentration
0.4mg/ml (varies by lot)
Applicable Applications for anti-SEPTIN2 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:200-1:1000
IHC-P: 1:25-1:100
Immunogen
Fusion protein of human SEPTIN2
Conjugation
Unconjugated
Modification
Unmodified
Preparation and Storage
Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Western Blot analysis of Septin 2 in HepG2 cells using Septin 2 Rabbit pAb at dilution 1/400)

Western Blot (WB) (Western Blot analysis of Septin 2 in HepG2 cells using Septin 2 Rabbit pAb at dilution 1/400)

Immunohistochemistry (IHC)

(Immunohistochemistry of Septin 2 in paraffin-embedded Human lung cancer tissue using Septin 2 Rabbit pAb at dilution 1/30. Predicted cell location: Cytoplasm and Nucleus)

Immunohistochemistry (IHC) (Immunohistochemistry of Septin 2 in paraffin-embedded Human lung cancer tissue using Septin 2 Rabbit pAb at dilution 1/30. Predicted cell location: Cytoplasm and Nucleus)
Related Product Information for anti-SEPTIN2 antibody
Filament-forming cytoskeletal GTPase. Forms a filamentous structure with SEPTIN12, SEPTIN6, SEPTIN2 and probably SEPTIN4 at the sperm annulus which is required for the structural integrity and motility of the sperm tail during postmeiotic differentiation (PubMed:25588830). Required for normal organization of the actin cytoskeleton. Plays a role in the biogenesis of polarized columnar-shaped epithelium by maintaining polyglutamylated microtubules, thus facilitating efficient vesicle transport, and by impeding MAP4 binding to tubulin. Required for the progression through mitosis. Forms a scaffold at the midplane of the mitotic splindle required to maintain CENPE localization at kinetochores and consequently chromosome congression. During anaphase, may be required for chromosome segregation and spindle elongation. Plays a role in ciliogenesis and collective cell movements. In cilia, required for the integrity of the diffusion barrier at the base of the primary cilium that prevents diffusion of transmembrane proteins between the cilia and plasma membranes: probably acts by regulating the assembly of the tectonic-like complex (also named B9 complex) by localizing TMEM231 protein. May play a role in the internalization of 2 intracellular microbial pathogens, Listeria monocytogenes and Shigella flexneri.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
41 KD
NCBI Official Full Name
septin-2
NCBI Official Synonym Full Names
septin 2
NCBI Official Symbol
SEPT2
NCBI Official Synonym Symbols
DIFF6; NEDD5; Pnutl3; hNedd5
NCBI Protein Information
septin-2; NEDD-5; neural precursor cell expressed, developmentally down-regulated 5; neural precursor cell expressed developmentally down-regulated protein 5
UniProt Protein Name
Septin-2
UniProt Gene Name
SEPT2
UniProt Synonym Gene Names
DIFF6; KIAA0158; NEDD5; NEDD-5
UniProt Entry Name
SEPT2_HUMAN

Uniprot Description

SEPT2: a member of the septin family of proteins. May be involved in the process of cytokinesis. Accumulates near the contractile ring from anaphase through telophase, and finally condenses into the midbody. In interphase and postmitotic cells, localized to fibrous or granular structures, depending on the growth state of the cell.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis; Cell development/differentiation; Cell cycle regulation; Hydrolase

Chromosomal Location of Human Ortholog: 2q37

Cellular Component: nucleoplasm; perinuclear region of cytoplasm; cytoplasm; nucleolus; exocyst; synapse; spindle; midbody; cell cortex; nucleus; actin cytoskeleton; cleavage furrow

Molecular Function: protein binding; GTP binding; protein complex scaffold; enzyme regulator activity

Biological Process: mitosis; smoothened signaling pathway; regulation of protein localization; cell division; regulation of catalytic activity; cilium biogenesis; regulation of L-glutamate transport; neurite development

Research Articles on SEPTIN2

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Product Notes

The SEPTIN2 sept2 (Catalog #AAA4760147) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Septin 2 Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Septin 2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:200-1:1000 IHC-P: 1:25-1:100. Researchers should empirically determine the suitability of the SEPTIN2 sept2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Septin 2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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