Rabbit anti-Human SEPP1 Polyclonal Antibody | anti-SEPP1 antibody

SEPP1, ID (SEPP1, SELP, Selenoprotein P) (APC)

Cat. Num. AAA6346109

• Gene Names: SELENOP; SeP; SELP; SEPP; SEPP1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SEPP1; Polyclonal Antibody; ID (SEPP1; SELP; Selenoprotein P) (APC); Selenoprotein P; anti-SEPP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-SEPP1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: SEPP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 232-262 amino acids from the Central region of human SEPP1.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(SEPP1 Antibody (Center) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the SEPP1 antibody detected the SEPP1 protein (arrow).)

Related Product Information for anti-SEPP1 antibody

This gene encodes a selenoprotein containing multiple selenocysteine (Sec) residues, which are encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenoprotein genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This selenoprotein is an extracellular glycoprotein, and is unusual in that it contains 10 Sec residues per polypeptide. It is a heparin-binding protein that appears to be associated with endothelial cells, and has been implicated to function as an antioxidant in the extracellular space. Several transcript variants, encoding either the same or different isoform, have been found for this gene.

Product Categories/Family for anti-SEPP1 antibody

Antibodies; Selenoprotein

References

Sun, W., et al. Br. J. Nutr. 104(9):1283-1287(2010). Roman, M., et al. Transl Res 156(4):242-250(2010). Meplan, C., et al. Carcinogenesis 31(6):1074-1079(2010). Davila, S., et al. Genes Immun. 11(3):232-238(2010). Takemoto, A.S., et al. Ethn Dis 20 (1 SUPPL 1), S1-S925 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 148277018
• NCBI GeneID: 6414
• NCBI Accession #: NP_001078955.1; NP_005401.3
• NCBI GenBank Nucleotide #: NM_001085486.2
• UniProt Accession #: P49908; Q6PD59; Q6PI43; Q6PI87; Q6PJF9
• Molecular Weight: 43,174 Da
• NCBI Official Full Name: selenoprotein P isoform 1
• NCBI Official Synonym Full Names: selenoprotein P
• NCBI Official Symbol: SELENOP
• NCBI Official Synonym Symbols: SeP; SELP; SEPP; SEPP1
• NCBI Protein Information: selenoprotein P
• UniProt Protein Name: Selenoprotein P
• Protein Family: Selenoprotein
• UniProt Gene Name: SELENOP
• UniProt Synonym Gene Names: SeP

NCBI Description

This gene encodes a selenoprotein that is predominantly expressed in the liver and secreted into the plasma. This selenoprotein is unique in that it contains multiple selenocysteine (Sec) residues per polypeptide (10 in human), and accounts for most of the selenium in plasma. It has been implicated as an extracellular antioxidant, and in the transport of selenium to extra-hepatic tissues via apolipoprotein E receptor-2 (apoER2). Mice lacking this gene exhibit neurological dysfunction, suggesting its importance in normal brain function. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. The mRNA for this selenoprotein contains two SECIS elements. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Feb 2017]

Uniprot Description

Might be responsible for some of the extracellular antioxidant defense properties of selenium or might be involved in the transport of selenium. May supply selenium to tissues such as brain and testis.