Rabbit anti-Human SENP6 Polyclonal Antibody | anti-SENP6 antibody

SENP6 Antibody

Cat. Num. AAA9408100

• Gene Names: SENP6; SSP1; SUSP1
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: SENP6; Polyclonal Antibody; SENP6 Antibody; EC 3.4.22.-; FKSG6; KIAA0797; Protease FKSG6; SEN6; anti-SENP6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total SENP6 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 1105

• Applicable Applications for anti-SENP6 antibody: Immunohistochemistry (IHC)
• Application Notes: Immunohistochemistry: 1:50~1:100
• Immunogen Type: Peptide
• Immunogen Description: Synthesized peptide derived from Internal of human SENP6.
• Target Name: SENP6
• Preparation and Storage: Store at -20 degree C

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using SENP6 antibody.)

Related Product Information for anti-SENP6 antibody

Protease that deconjugates SUMO1, SUMO2 and SUMO3 from targeted proteins. Processes preferentially poly-SUMO2 and poly-SUMO3 chains, but does not efficiently process SUMO1, SUMO2 and SUMO3 precursors. Deconjugates SUMO1 from RXRA, leading to transcriptional activation. Involved in chromosome alignment and spindle assembly, by regulating the kinetochore CENPH-CENPI-CENPK complex. Desumoylates PML and CENPI, protecting them from degradation by the ubiquitin ligase RNF4, which targets polysumoylated proteins for proteasomal degradation. Desumoylates also RPA1, thus preventing recruitment of RAD51 to the DNA damage foci to initiate DNA repair through homologous recombination.Dong Chen, Blood (ASH Annual Meeting Abstracts), Nov 2005; 106: 4188.Debaditya Mukhopadhyay, J. Cell Biol., Sep 2006; 174: 939 - 949. Jinke Cheng, Mol. Cell. Biol., Jul 2004; 24: 6021 - 6028. Marius C. Jones, PNAS, Oct 2006; 103: 16272 - 16277.

Product Categories/Family for anti-SENP6 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 156105703
• NCBI GeneID: 26054
• NCBI Accession #: NP_001093879.1
• NCBI GenBank Nucleotide #: NM_001100409.2
• UniProt Accession #: Q9GZR1; O94891; Q5VUL3; Q5VUL4; Q8TBY4; Q9UJV5; A6NNY9
• Molecular Weight: 125,330 Da
• NCBI Official Full Name: sentrin-specific protease 6 isoform 2
• NCBI Official Synonym Full Names: SUMO1/sentrin specific peptidase 6
• NCBI Official Symbol: SENP6
• NCBI Official Synonym Symbols: SSP1; SUSP1
• NCBI Protein Information: sentrin-specific protease 6
• UniProt Protein Name: Sentrin-specific protease 6
• Protein Family: Sentrin-specific protease
• UniProt Gene Name: SENP6
• UniProt Synonym Gene Names: KIAA0797; SSP1; SUSP1
• UniProt Entry Name: SENP6_HUMAN

NCBI Description

Ubiquitin-like molecules (UBLs), such as SUMO1 (UBL1; MIM 601912), are structurally related to ubiquitin (MIM 191339) and can be ligated to target proteins in a similar manner as ubiquitin. However, covalent attachment of UBLs does not result in degradation of the modified proteins. SUMO1 modification is implicated in the targeting of RANGAP1 (MIM 602362) to the nuclear pore complex, as well as in stabilization of I-kappa-B-alpha (NFKBIA; MIM 164008) from degradation by the 26S proteasome. Like ubiquitin, UBLs are synthesized as precursor proteins, with 1 or more amino acids following the C-terminal glycine-glycine residues of the mature UBL protein. Thus, the tail sequences of the UBL precursors need to be removed by UBL-specific proteases, such as SENP6, prior to their conjugation to target proteins (Kim et al., 2000 [PubMed 10799485]). SENPs also display isopeptidase activity for deconjugation of SUMO-conjugated substrates (Lima and Reverter, 2008 [PubMed 18799455]).[supplied by OMIM, Jun 2009]

Uniprot Description

SENP6: Protease that deconjugates SUMO1, SUMO2 and SUMO3 from targeted proteins. Processes preferentially poly-SUMO2 and poly- SUMO3 chains, but does not efficiently process SUMO1, SUMO2 and SUMO3 precursors. Deconjugates SUMO1 from RXRA, leading to transcriptional activation. Involved in chromosome alignment and spindle assembly, by regulating the kinetochore CENPH-CENPI-CENPK complex. Desumoylates PML and CENPI, protecting them from degradation by the ubiquitin ligase RNF4, which targets polysumoylated proteins for proteasomal degradation. Desumoylates also RPA1, thus preventing recruitment of RAD51 to the DNA damage foci to initiate DNA repair through homologous recombination. Belongs to the peptidase C48 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; EC 3.4.22.68

Chromosomal Location of Human Ortholog: 6q14.1

Cellular Component: nucleoplasm; cytoplasm

Molecular Function: protein binding; SUMO-specific protease activity

Biological Process: protein desumoylation; protein sumoylation; proteolysis