Western Blot (WB)
(SEMA3G Antibody (Center) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the SEMA3G antibody detected the SEMA3G protein (arrow).)
Rabbit anti-Human SEMA3G Polyclonal Antibody | anti-SEMA3G antibody
SEMA3G, ID (SEMA3G, Semaphorin-3G, Semaphorin sem2) (Biotin)
Gene Names
SEMA3G; sem2
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
SEMA3G; Polyclonal Antibody; ID (SEMA3G; Semaphorin-3G; Semaphorin sem2) (Biotin); Semaphorin sem2; anti-SEMA3G antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Sequence Length
782
Applicable Applications for anti-SEMA3G antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:50-100
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
SEMA3G antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 223-252 amino acids from the Central region of human SEMA3G.
Conjugate
Biotin
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(SEMA3G Antibody (Center) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the SEMA3G antibody detected the SEMA3G protein (arrow).)
Western Blot (WB)
(SEMA3G Antibody (Center) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the SEMA3G antibody detected the SEMA3G protein (arrow).)
Immunohistochemistry (IHC)
(SEMA3G Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SEMA3G Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(SEMA3G Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SEMA3G Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-SEMA3G antibody
SEMA3G has chemorepulsive activities for sympathetic axons. Ligand of NRP2 (By similarity).
Product Categories/Family for anti-SEMA3G antibody
References
Bailey, S.D., et al. Diabetes Care (2010). In press: Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Karayan-Tapon, L., et al. Br. J. Cancer 99(7):1153-1160(2008). Kigel, B., et al. PLoS ONE 3 (9), E3287 (2008): Rikova, K., et al. Cell 131(6):1190-1203(2007).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
semaphorin-3G
NCBI Official Synonym Full Names
semaphorin 3G
NCBI Official Symbol
SEMA3G
NCBI Official Synonym Symbols
sem2
NCBI Protein Information
semaphorin-3G
UniProt Protein Name
Semaphorin-3G
Protein Family
UniProt Gene Name
SEMA3G
UniProt Entry Name
SEM3G_HUMAN
NCBI Description
The transcription of this gene is activated by PPAR-gamma, and the resulting protein product plays a role in endothelial cell migration. Expression of this gene also inhibits tumor cell migration and invasion. [provided by RefSeq, Jul 2016]
Uniprot Description
SEMA3G: a secreted protein that has chemorepulsive activities for sympathetic axons. A high-affinity ligand of NRP2.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 3p21.1
Cellular Component: membrane
Molecular Function: receptor activity
Biological Process: multicellular organismal development
Research Articles on SEMA3G
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Product Notes
The SEMA3G sema3g (Catalog #AAA6345989) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SEMA3G, ID (SEMA3G, Semaphorin-3G, Semaphorin sem2) (Biotin) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SEMA3G can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:50-100 ELISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the SEMA3G sema3g for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SEMA3G, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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