Rabbit SEH / EPHX2 Polyclonal Antibody | anti-SEH antibody

Rabbit Polyclonal to Human SEH / EPHX2

Cat. Num. AAA243333

• Gene Names: EPHX2; CEH; SEH
• Reactivity: Human; Predicted Reactivity: Gorilla, Orangutan (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry
• Purity: Immunoaffinity Purified
• Synonyms: SEH / EPHX2; Polyclonal Antibody; Rabbit Polyclonal to Human SEH / EPHX2; Anti-SEH / EPHX2 Antibody (N-Terminus) IHC-plus; EPHX2; CEH; Epoxide hydratase; Epoxide hydrolase; soluble; Epoxide hydrolase 2; cytosolic; SEH; Soluble epoxide hydrolase; Human SEH; anti-SEH antibody

• Host: Rabbit
• Reactivity: Human; Predicted Reactivity: Gorilla, Orangutan (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human EPHX2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.1% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 555

• Applicable Applications for anti-SEH antibody: Immunohistochemistry (IHC - Paraffin)
• Application Notes: IHC-P (5 ug/ml)
• Target Species: Human
• Immunogen Description: Synthetic 19 amino acid peptide from Near the N-terminus of human EPHX2. Percent identity with other species by BLAST analysis: Human (100%); Gorilla, Orangutan (95%); Gibbon, Monkey (89%); Marmoset (84%).
• Immunogen Type: Synthetic peptide
• Immunogen: SEH / EPHX2 antibody was raised against synthetic 19 amino acid peptide from Near the N-terminus of human EPHX2. Percent identity with other species by BLAST analysis: Human (100%); Gorilla, Orangutan (95%); Gibbon, Monkey (89%); Marmoset (84%).
• Antigen Modification: N-Terminus
• Preparation and Storage: Long term: -70 degree C; Short term: +4 degree C

Immunohistochemistry (IHC)

(Anti-EPHX2 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Related Product Information for anti-SEH antibody

Soluble Epoxide Hydrolase (sEH) also known as cytosolic Epoxide Hydrolase is a phase I xenobiotic-metabolizing enzyme that converts epoxyeicosatrienoic acids (EETs) into their corresponding dihydroxyeicosatrienoic acids (DHETs). EETs are thought to be involved in vascular tone, ion channel regulation, mitogenesis and cell signaling.

NCBI and Uniprot Product Information

• NCBI GI #: 27597073
• NCBI GeneID: 2053
• NCBI Accession #: NP_001970.2
• NCBI GenBank Nucleotide #: NM_001979.5
• UniProt Accession #: P34913; Q16764; Q9HBJ1; Q9HBJ2; B2Z3B1; B3KTU8; B3KUA0; G3V134; J3KPH7
• Molecular Weight: 55,626 Da
• NCBI Official Full Name: bifunctional epoxide hydrolase 2 isoform a
• NCBI Official Synonym Full Names: epoxide hydrolase 2, cytoplasmic
• NCBI Official Symbol: EPHX2
• NCBI Official Synonym Symbols: CEH; SEH
• NCBI Protein Information: bifunctional epoxide hydrolase 2; epoxide hydratase; epoxide hydrolase, soluble; epoxide hydrolase 2, cytosolic
• UniProt Protein Name: Bifunctional epoxide hydrolase 2
• UniProt Gene Name: EPHX2
• UniProt Synonym Gene Names: CEH; SEH
• UniProt Entry Name: HYES_HUMAN

NCBI Description

This gene encodes a member of the epoxide hydrolase family. The protein, found in both the cytosol and peroxisomes, binds to specific epoxides and converts them to the corresponding dihydrodiols. Mutations in this gene have been associated with familial hypercholesterolemia. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2012]

Uniprot Description

EPHX2: Bifunctional enzyme. The C-terminal domain has epoxide hydrolase activity and acts on epoxides (alkene oxides, oxiranes) and arene oxides. Plays a role in xenobiotic metabolism by degrading potentially toxic epoxides. Also determines steady-state levels of physiological mediators. The N-terminal domain has lipid phosphatase activity, with the highest activity towards threo- 9,10-phosphonooxy-hydroxy-octadecanoic acid, followed by erythro- 9,10-phosphonooxy-hydroxy-octadecanoic acid, 12-phosphonooxy- octadec-9Z-enoic acid, 12-phosphonooxy-octadec-9E-enoic acid, and p-nitrophenyl phospate. Belongs to the AB hydrolase superfamily. Epoxide hydrolase family.

Protein type: EC 3.3.2.10; Lipid Metabolism - arachidonic acid; EC 3.1.3.76; Hydrolase

Chromosomal Location of Human Ortholog: 8p21

Cellular Component: cytoplasm; peroxisome; cytosol

Molecular Function: toxin binding; protein homodimerization activity; lipid-phosphate phosphatase activity; epoxide hydrolase activity; phosphoric monoester hydrolase activity; magnesium ion binding; lipid phosphatase activity; receptor binding

Biological Process: cellular calcium ion homeostasis; cholesterol homeostasis; dephosphorylation; xenobiotic metabolic process; regulation of blood pressure; response to toxin; arachidonic acid metabolic process; epoxygenase P450 pathway; stilbene catabolic process; drug metabolic process; inflammatory response; positive regulation of vasodilation; phospholipid dephosphorylation

Disease: Hypercholesterolemia, Familial