Rabbit anti-Human Securin Polyclonal Antibody | anti-PTTG1 antibody

Securin, NT (PTTG1, Pituitary Tumor-transforming Gene 1 Protein, hPTTG, Tumor-transforming Protein 1, Esp1-associated Protein, TUTR1, PTTG, EAP1) (MaxLight 550)

Cat. Num. AAA6498500

• Gene Names: PTTG1; EAP1; PTTG; HPTTG; TUTR1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: Securin; Polyclonal Antibody; NT (PTTG1; Pituitary Tumor-transforming Gene 1 Protein; hPTTG; Tumor-transforming Protein 1; Esp1-associated Protein; TUTR1; PTTG; EAP1) (MaxLight 550); anti-PTTG1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PTTG1.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.

• Applicable Applications for anti-PTTG1 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human PTTG1 (KLH).
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of PTTG1 antibody (N-term) in Ramos cell line lysates (35ug/lane). PTTG1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human testis tissue with PTTG1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-PTTG1 antibody

Antibodies; Cell Division Cycle (CDC)

References

1. Li, T., J Genet Genomics 36(6), 335-342 (2009). 2. Yan, S., Cancer Res. 69(8), 3283-3290 (2009). 3. Chesnokova, V., Horm. Res. 71 SUPPL 2, 82-87 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4758980
• NCBI GeneID: 9232
• NCBI Accession #: NP_004210
• NCBI GenBank Nucleotide #: NM_004219.3
• UniProt Accession #: O95997
• Molecular Weight: 24.1 kDa (222aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: securin
• NCBI Official Synonym Full Names: pituitary tumor-transforming 1
• NCBI Official Symbol: PTTG1
• NCBI Official Synonym Symbols: EAP1; PTTG; HPTTG; TUTR1
• NCBI Protein Information: securin
• UniProt Protein Name: Securin
• Protein Family: Securin
• UniProt Gene Name: PTTG1
• UniProt Synonym Gene Names: EAP1; PTTG; TUTR1; Tumor-transforming protein 1; hPTTG
• UniProt Entry Name: PTTG1_HUMAN

NCBI Description

The encoded protein is a homolog of yeast securin proteins, which prevent separins from promoting sister chromatid separation. It is an anaphase-promoting complex (APC) substrate that associates with a separin until activation of the APC. The gene product has transforming activity in vitro and tumorigenic activity in vivo, and the gene is highly expressed in various tumors. The gene product contains 2 PXXP motifs, which are required for its transforming and tumorigenic activities, as well as for its stimulation of basic fibroblast growth factor expression. It also contains a destruction box (D box) that is required for its degradation by the APC. The acidic C-terminal region of the encoded protein can act as a transactivation domain. The gene product is mainly a cytosolic protein, although it partially localizes in the nucleus. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Sep 2013]

Uniprot Description

Securin: Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however not limited to a blocking activity, since it is required to activate ESPL1. Negatively regulates the transcriptional activity and related apoptosis activity of TP53. The negative regulation of TP53 may explain the strong transforming capability of the protein when it is overexpressed. May also play a role in DNA repair via its interaction with Ku, possibly by connecting DNA damage-response pathways with sister chromatid separation. Belongs to the securin family.

Protein type: Oncoprotein; Cell cycle regulation

Chromosomal Location of Human Ortholog: 5q35.1

Cellular Component: cytoplasm; cytosol; nucleus

Molecular Function: protein binding; cysteine protease inhibitor activity; SH3 domain binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; mitosis; regulation of transcription, DNA-dependent; cell division; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; spermatogenesis; mitotic cell cycle; DNA repair; chromosome organization and biogenesis; chromosome segregation