Western Blot (WB)
(Western Blot: Sample: Lane1: Rat Heart Tissue; Lane2: Rat Brain Tissue.)
Rabbit anti-Rat Secreted Frizzled Related Protein 1 (SFRP1) Polyclonal Antibody | anti-SFRP1 antibody
Polyclonal Antibody to Secreted Frizzled Related Protein 1 (SFRP1)
Gene Names
Sfrp1; sFRP-1
Reactivity
Rat
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Secreted Frizzled Related Protein 1 (SFRP1); Polyclonal Antibody; Polyclonal Antibody to Secreted Frizzled Related Protein 1 (SFRP1); anti-SFRP1 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against SFRP1. It has been selected for its ability to recognize SFRP1 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence Length
314
Applicable Applications for anti-SFRP1 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Lane1: Rat Heart Tissue; Lane2: Rat Brain Tissue.)
Western Blot (WB)
(Western Blot: Sample: Lane1: Rat Heart Tissue; Lane2: Rat Brain Tissue.)
Western Blot (WB)
(Western Blot: Sample: Recombinant SFRP1, Rat.)
Western Blot (WB)
(Western Blot: Sample: Recombinant SFRP1, Rat.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Rat Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Rat Tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
18,072 Da
NCBI Official Full Name
Secreted frizzled-related protein 1
NCBI Official Synonym Full Names
secreted frizzled-related protein 1
NCBI Official Symbol
Sfrp1
NCBI Official Synonym Symbols
sFRP-1
NCBI Protein Information
secreted frizzled-related protein 1
UniProt Protein Name
Secreted frizzled-related protein 1
Protein Family
UniProt Gene Name
Sfrp1
UniProt Synonym Gene Names
sFRP-1
NCBI Description
a secreted protein involved in binding Wnt and antagonizing Wnt signaling [RGD, Feb 2006]
Uniprot Description
Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP1 decreases intracellular beta-catenin levels (). Has antiproliferative effects on vascular cells, in vitro and in vivo, and can induce, in vivo, an angiogenic response. In vascular cell cycle, delays the G1 phase and entry into the S phase. In kidney development, inhibits tubule formation and bud growth in metanephroi. Inhibits WNT1/WNT4-mediated TCF-dependent transcription.
Research Articles on SFRP1
Similar Products
Product Notes
The SFRP1 sfrp1 (Catalog #AAA2006049) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Secreted Frizzled Related Protein 1 (SFRP1) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Secreted Frizzled Related Protein 1 (SFRP1) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the SFRP1 sfrp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Secreted Frizzled Related Protein 1 (SFRP1), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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