Rabbit SEC62 Polyclonal Antibody | anti-SEC62 antibody

SEC62 Antibody

Cat. Num. AAA150395

• Gene Names: SEC62; HTP1; TP-1; Dtrp1; TLOC1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: SEC62 Antibody is affinity chromatography purified via peptide column.
• Synonyms: SEC62; Polyclonal Antibody; SEC62 Antibody; HTP1; TP-1; Dtrp1; TLOC1; Translocation protein SEC62; Translocation protein 1; SEC62 homolog (S. cerevisiae); anti-SEC62 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SEC62 antibody is predicted to not cross-react with other SEC proteins. Multiple isoforms of SEC62 are known to exist.
• Purity/Purification: SEC62 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 399

• Applicable Applications for anti-SEC62 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: SEC62 antibody can be used for detection of SEC62 by Western blot at 0.5 - 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: SEC62 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SEC62.
• Buffer: SEC62 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: SEC62 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of SEC62 in rat brain tissue lysate with SEC62 antibody at (A) 0.5 and (B) 1 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of SEC62 in mouse brain tissue with SEC62 antibody at 5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of SEC62 in mouse brain tissue with SEC62 antibody at 20 μg/mL.)

Related Product Information for anti-SEC62 antibody

SEC62 Antibody: SEC62 is an integral membrane protein located in the rough endoplasmic reticulum (ER) and is part of the SEC61-SEC62-SEC63 complex that is the central component of the protein translocation apparatus of the ER membrane. It is speculated that SEC61-SEC62-SEC63 may perform post-translational protein translocation into the ER and might also perform the backward transport of ER proteins that are subject to the ubiquitin-proteasome-dependent degradation pathway. Silencing of this gene with RNAi dramatically reduced the migration and invasive potential of numerous tumor cell lines, suggesting that it may be an attractive target for therapy of various tumors.

NCBI and Uniprot Product Information

• NCBI GI #: 4507525
• NCBI GeneID: 7095
• NCBI Accession #: NP_003253
• NCBI GenBank Nucleotide #: NM_003262.3
• UniProt Accession #: Q99442; O00682; O00729; D3DNQ0
• Molecular Weight: 45,862 Da
• NCBI Official Full Name: translocation protein SEC62
• NCBI Official Synonym Full Names: SEC62 homolog (S. cerevisiae)
• NCBI Official Symbol: SEC62
• NCBI Official Synonym Symbols: HTP1; TP-1; Dtrp1; TLOC1
• NCBI Protein Information: translocation protein SEC62; hTP-1; translocation protein 1; membrane protein SEC62, S.cerevisiae, homolog of
• UniProt Protein Name: Translocation protein SEC62
• Protein Family: Translocation protein
• UniProt Gene Name: SEC62
• UniProt Synonym Gene Names: TLOC1; TP-1; hTP-1
• UniProt Entry Name: SEC62_HUMAN

NCBI Description

The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. The protein encoded by this gene and SEC63 protein are found to be associated with ribosome-free SEC61 complex. It is speculated that Sec61-Sec62-Sec63 may perform post-translational protein translocation into the ER. The Sec61-Sec62-Sec63 complex might also perform the backward transport of ER proteins that are subject to the ubiquitin-proteasome-dependent degradation pathway. The encoded protein is an integral membrane protein located in the rough ER. [provided by RefSeq, Jul 2008]

Uniprot Description

SEC62: a multi-pass membrane protein and component of the Sec61 complex of the rough endoplasmic reticulum membrane. The Sec61 complex may perform post-translational protein translocation into the ER and backward transport of ER proteins that are subject to the ubiquitin-proteasome-dependent degradation pathway.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Channel, misc.; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 3q26.2

Cellular Component: intermediate filament cytoskeleton; endoplasmic reticulum membrane; rough endoplasmic reticulum; membrane; endoplasmic reticulum; integral to membrane

Molecular Function: receptor activity; protein transporter activity

Biological Process: posttranslational protein targeting to membrane; cotranslational protein targeting to membrane