Immunofluorescence (IF)
(Immunofluorescence analysis of methanol-fixed HeLa, using SDHB antibody at 1:200 dilution.)
Rabbit SDHB Polyclonal Antibody | anti-SDHB antibody
SDHB Antibody
Applications
Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Antigen-Affinity Chromatography
Synonyms
SDHB; Polyclonal Antibody; SDHB Antibody; Affinity Purified Rabbit Polyclonal SDHB Antibody; Polyclonal SDHB antibody; Anti-SDHB antibody; FLJ92337 antibody; SDH2 antibody; SDH1 antibody; IP antibody; SDH antibody; SDHB antibody; PGL4 antibody; SDHIP antibody; anti-SDHB antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen-Affinity Chromatography
Form/Format
Supplied as a concentrated soloution containing 1X PBS, 40% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
1 mg/ml (varies by lot)
Sequence Length
319
Applicable Applications for anti-SDHB antibody
Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes
IF: 1:100-1:200
WB: 1:500-1:3000
WB: 1:500-1:3000
Immunogen
SDHB antibody was raised in rabbit using a synthetic peptide corresponding to a region within amino acids 221 and 280 of SDHB
Cross-Reactivity
Human
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Immunofluorescence (IF)
(Immunofluorescence analysis of methanol-fixed HeLa, using SDHB antibody at 1:200 dilution.)
Immunofluorescence (IF)
(Immunofluorescence analysis of methanol-fixed HeLa, using SDHB antibody at 1:200 dilution.)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A:MOLT4) using a 12% SDS PAGE gel and SDHB antibody at a dilution of 1:1000)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A:MOLT4) using a 12% SDS PAGE gel and SDHB antibody at a dilution of 1:1000)
Related Product Information for anti-SDHB antibody
Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme. Sporadic and familial mutations in this gene result in paragangliomas and pheochromocytoma, and support a link between mitochondrial dysfunction and tumorigenesis.
Product Categories/Family for anti-SDHB antibody
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
32 kDa
NCBI Official Full Name
SdhB
Protein Family
Similar Products
Product Notes
The SDHB (Catalog #AAA5311962) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's SDHB can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB). IF: 1:100-1:200 WB: 1:500-1:3000. Researchers should empirically determine the suitability of the SDHB for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SDHB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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