Goat SDF4 Polyclonal Antibody | anti-SDF4 antibody

Goat anti-SDF4 (aa161-175) Antibody

Cat. Num. AAA422557

• Gene Names: SDF4; Cab45; SDF-4
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: SDF4; Polyclonal Antibody; Goat anti-SDF4 (aa161-175) Antibody; stromal cell derived factor 4; Cab45; 45 kDa calcium-binding protein; OTTHUMP00000001754; SDF-4; calcium binding protein; stromal cell-derived factor 4; SDF4 antibody; stromal cell derived factor 4 antibody; Cab45 antibody; 45 kDa calcium-binding protein antibody; OTTHUMP00000001754 antibody; SDF-4 antibody; calcium binding protein antibody; stromal cell-derived factor 4 antibody; SDF4 (aa161-175); anti-SDF4 antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: EYKVKFLASKGHSEK
• Sequence Length: 348

• Applicable Applications for anti-SDF4 antibody: Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 64000.; Immunohistochemistry: In paraffin embedded Human Kidney shows preferential staining in the brush borders of epithelial cells. Recommended concentration, 5-10ug/ml.; Western Blot: Approx 45kDa band observed in lysates of cell line Jurkat (calculated MW of 41.8kDa according to NP_057260.2). Recommended concentration: 0.1-0.3ug/ml.
• Immunogen: Peptide with sequence C-EYKVKFLASKGHSEK, from the internal region of the protein sequence according to NP_057631.1; NP_057260.2.
• Epitope: Internal region
• Note: This antibody is expected to recognize both reported isoforms (NP_057631.1; NP_057260.2).
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.1ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Immunohistochemistry (IHC)

((5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)

NCBI and Uniprot Product Information

• NCBI GI #: 7706573
• NCBI GeneID: 51150
• NCBI Accession #: NP_057631.1; NP_057260.2
• NCBI GenBank Nucleotide #: NM_016547.2
• Molecular Weight: 39,608 Da
• NCBI Official Full Name: 45 kDa calcium-binding protein isoform 1
• NCBI Official Synonym Full Names: stromal cell derived factor 4
• NCBI Official Symbol: SDF4
• NCBI Official Synonym Symbols: Cab45; SDF-4
• NCBI Protein Information: 45 kDa calcium-binding protein
• UniProt Protein Name: 45 kDa calcium-binding protein
• Protein Family: 45 kDa calcium-binding protein
• UniProt Gene Name: SDF4
• UniProt Synonym Gene Names: CAB45; Cab45; SDF-4
• UniProt Entry Name: CAB45_HUMAN

NCBI Description

This gene encodes a stromal cell derived factor that is a member of the CREC protein family. The encoded protein contains six EF-hand motifs and calcium-binding motifs. This protein localizes to the Golgi lumen and may be involved in regulating calcium dependent cellular activities. [provided by RefSeq, Sep 2011]

Uniprot Description

SDF4: May regulate calcium-dependent activities in the endoplasmic reticulum lumen or post-ER compartment. Belongs to the CREC family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Calcium-binding

Chromosomal Location of Human Ortholog: 1p36.33

Cellular Component: cytoplasm; Golgi apparatus; Golgi lumen; late endosome; membrane; plasma membrane

Molecular Function: calcium ion binding; identical protein binding; protein binding

Biological Process: calcium ion-dependent exocytosis; cerebellum development; fat cell differentiation; response to ethanol; UV protection